A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid)

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  • Kasper Kannegård Karlsen, NAC, Institut for Fysik og Kemi, Danmark
  • Anders Hauge Okholm, Danmark
  • Jørgen Kjems
  • Jesper Wengel, Institut for Fysik, Kemi og Farmaci, Danmark
A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization of a fully complementary RNA target was accompanied by a pyrene excimer emission increase of more than 900%, and detection of RNA in living cells was demonstrated.
OriginalsprogEngelsk
TidsskriftBioorganic & Medicinal Chemistry
Vol/bind21
Nummer20
Sider (fra-til)6186-6190
Antal sider6
ISSN0968-0896
StatusUdgivet - 15 okt. 2013

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