A Promiscuity Locus Confers Lotus burttii Nodulation with Rhizobia from Five Different Genera

TY - JOUR

T1 - A Promiscuity Locus Confers Lotus burttii Nodulation with Rhizobia from Five Different Genera

AU - Zarrabian, Mohammad

AU - Montiel, Jesús

AU - Sandal, Niels

AU - Ferguson, Shaun

AU - Jin, Haojie

AU - Lin, Yen Yu

AU - Klingl, Verena

AU - Marín, Macarena

AU - James, Euan K.

AU - Parniske, Martin

AU - Stougaard, Jens

AU - Andersen, Stig U.

PY - 2022/11

Y1 - 2022/11

N2 - Legumes acquire access to atmospheric nitrogen through nitrogen fixation by rhizobia in root nodules. Rhizobia are soil-dwelling bacteria and there is a tremendous diversity of rhizobial species in different habitats. Fromthe legume perspective, host range is a compromise between the ability to colonize new habitats, in which the preferred symbiotic partner may be absent, and guarding against infection by suboptimal nitrogen fixers. Here, we investigate natural variation in rhizobial host range across Lotus species. We find that Lotus burttii is considerably more promiscuous than Lotus japonicus, represented by theGifu accession, in its interactions with rhizobia.This promiscuity allows Lotus burttii to form nodules with Mesorhizobium, Rhizobium, Sinorhizobium, Bradyrhizobium, and Allorhizobium species that represent five distinct genera. Using recombinant inbred lines,we havemapped the Gifu/burttii promiscuity quantitative trait loci (QTL) to the same genetic locus regardless of rhizobial genus, suggesting a general genetic mechanism for symbiont-range expansion. The Gifu/burttii QTL now provides an opportunity for genetic and mechanistic understanding of promiscuous legume-rhizobia interactions.

AB - Legumes acquire access to atmospheric nitrogen through nitrogen fixation by rhizobia in root nodules. Rhizobia are soil-dwelling bacteria and there is a tremendous diversity of rhizobial species in different habitats. Fromthe legume perspective, host range is a compromise between the ability to colonize new habitats, in which the preferred symbiotic partner may be absent, and guarding against infection by suboptimal nitrogen fixers. Here, we investigate natural variation in rhizobial host range across Lotus species. We find that Lotus burttii is considerably more promiscuous than Lotus japonicus, represented by theGifu accession, in its interactions with rhizobia.This promiscuity allows Lotus burttii to form nodules with Mesorhizobium, Rhizobium, Sinorhizobium, Bradyrhizobium, and Allorhizobium species that represent five distinct genera. Using recombinant inbred lines,we havemapped the Gifu/burttii promiscuity quantitative trait loci (QTL) to the same genetic locus regardless of rhizobial genus, suggesting a general genetic mechanism for symbiont-range expansion. The Gifu/burttii QTL now provides an opportunity for genetic and mechanistic understanding of promiscuous legume-rhizobia interactions.

KW - genetic mapping

KW - host range

KW - Lotus

KW - rhizobia

KW - symbiotic nitrogen fixation

UR - http://www.scopus.com/inward/record.url?scp=85142918530&partnerID=8YFLogxK

U2 - 10.1094/MPMI-06-22-0124-R

DO - 10.1094/MPMI-06-22-0124-R

M3 - Journal article

C2 - 35852471

AN - SCOPUS:85142918530

SN - 0894-0282

VL - 35

SP - 1006

EP - 1017

JO - Molecular plant-microbe interactions

JF - Molecular plant-microbe interactions

IS - 11

ER -