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A novel wheat q’ allele identified by forward genetic in silico TILLING

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A novel wheat q’ allele identified by forward genetic in silico TILLING. / Madsen, Claus Krogh; Brinch-Pedersen, Henrik.

I: Journal of Plant Physiology, Bind 251, 153221, 08.2020.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{45c5ad49abd0404fa758fcb9682fe8c9,
title = "A novel wheat q{\textquoteright} allele identified by forward genetic in silico TILLING",
abstract = "The major wheat domestication allele Q (encoding an APETALA2 like transcription factor) is responsible for the free threshing and square-headed spikes of modern wheat. Wild type q and null q{\textquoteright} alleles cause a reversal to the speltoid phenotype. Q pleiotropically affects additional yield and quality traits so genetic variation in Q and its interaction partners remain important for crop improvement. Here we report the discovery of a novel q{\textquoteright} allele that contribute to the molecular characterization of Q. This induced mutation cause an Ala→Thr substitution at a highly conserved position in the first of two APETALA2 like domains of the Q protein. The mutation correlates with a speltoid phenotype as expected for a loss of Q function. Thus, it is demonstrated that the first APETALA2 like domain is essential for Q function. The phenotype was discovered in a mutant from an in silico TILLING population and the causative mutation could be identified in the existing sequence data. This report therefore provides an example of a forward genetic application of in silico TILLING.",
keywords = "Q gene, Spelt wheat, APETALA2, In silicoTILLING, Domstication",
author = "Madsen, {Claus Krogh} and Henrik Brinch-Pedersen",
year = "2020",
month = aug,
doi = "10.1016/j.jplph.2020.153221",
language = "English",
volume = "251",
journal = "Journal of Plant Physiology",
issn = "0176-1617",
publisher = "Elsevier GmbH - Urban und Fischer",

}

RIS

TY - JOUR

T1 - A novel wheat q’ allele identified by forward genetic in silico TILLING

AU - Madsen, Claus Krogh

AU - Brinch-Pedersen, Henrik

PY - 2020/8

Y1 - 2020/8

N2 - The major wheat domestication allele Q (encoding an APETALA2 like transcription factor) is responsible for the free threshing and square-headed spikes of modern wheat. Wild type q and null q’ alleles cause a reversal to the speltoid phenotype. Q pleiotropically affects additional yield and quality traits so genetic variation in Q and its interaction partners remain important for crop improvement. Here we report the discovery of a novel q’ allele that contribute to the molecular characterization of Q. This induced mutation cause an Ala→Thr substitution at a highly conserved position in the first of two APETALA2 like domains of the Q protein. The mutation correlates with a speltoid phenotype as expected for a loss of Q function. Thus, it is demonstrated that the first APETALA2 like domain is essential for Q function. The phenotype was discovered in a mutant from an in silico TILLING population and the causative mutation could be identified in the existing sequence data. This report therefore provides an example of a forward genetic application of in silico TILLING.

AB - The major wheat domestication allele Q (encoding an APETALA2 like transcription factor) is responsible for the free threshing and square-headed spikes of modern wheat. Wild type q and null q’ alleles cause a reversal to the speltoid phenotype. Q pleiotropically affects additional yield and quality traits so genetic variation in Q and its interaction partners remain important for crop improvement. Here we report the discovery of a novel q’ allele that contribute to the molecular characterization of Q. This induced mutation cause an Ala→Thr substitution at a highly conserved position in the first of two APETALA2 like domains of the Q protein. The mutation correlates with a speltoid phenotype as expected for a loss of Q function. Thus, it is demonstrated that the first APETALA2 like domain is essential for Q function. The phenotype was discovered in a mutant from an in silico TILLING population and the causative mutation could be identified in the existing sequence data. This report therefore provides an example of a forward genetic application of in silico TILLING.

KW - Q gene

KW - Spelt wheat

KW - APETALA2

KW - In silicoTILLING

KW - Domstication

U2 - 10.1016/j.jplph.2020.153221

DO - 10.1016/j.jplph.2020.153221

M3 - Journal article

C2 - 32590253

VL - 251

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

M1 - 153221

ER -