TY - JOUR
T1 - A New High-Throughput Screening Method for Phages
T2 - Enabling Crude Isolation and Fast Identification of Diverse Phages with Therapeutic Potential
AU - Olsen, Nikoline S.
AU - Hendriksen, Niels Bohse
AU - Hansen, Lars Hestbjerg
AU - Kot, Witold
PY - 2020/9
Y1 - 2020/9
N2 - Bacteriophage therapy and application of phages for biocontrol necessitate acquisition of suitable phages. The exclusivity of phage–host relations and the risk of phage resistance instigate a need to rapidly isolate and characterize novel phages and continually build sizeable phage libraries. Current methods for phage isolation are both laborious and time consuming, suitable for the isolation of a limited number of phages. The high-throughput screening method for phages upscales and organizes enrichment of phages for fast isolation and identification of potentially hundreds of distinct phages against single hosts. This enables screening of hundreds of samples, in multiple simultaneous setups with varying parameters, increasing the likelihood of isolating multiple distinct phages specific for the given conditions. The efficiency of the method is emphasized by our screening of 200 environmental samples, resulting in the identification of an abundance of unique phage species virulent to Escherichia coli, Salmonella enterica, Enterococcus faecalis, and Pseudomonas aeruginosa.
AB - Bacteriophage therapy and application of phages for biocontrol necessitate acquisition of suitable phages. The exclusivity of phage–host relations and the risk of phage resistance instigate a need to rapidly isolate and characterize novel phages and continually build sizeable phage libraries. Current methods for phage isolation are both laborious and time consuming, suitable for the isolation of a limited number of phages. The high-throughput screening method for phages upscales and organizes enrichment of phages for fast isolation and identification of potentially hundreds of distinct phages against single hosts. This enables screening of hundreds of samples, in multiple simultaneous setups with varying parameters, increasing the likelihood of isolating multiple distinct phages specific for the given conditions. The efficiency of the method is emphasized by our screening of 200 environmental samples, resulting in the identification of an abundance of unique phage species virulent to Escherichia coli, Salmonella enterica, Enterococcus faecalis, and Pseudomonas aeruginosa.
KW - Bacteriophage
KW - high-throughput
KW - phage isolation
KW - phage therapy
KW - wastewater
UR - http://www.scopus.com/inward/record.url?scp=85098558990&partnerID=8YFLogxK
U2 - 10.1089/phage.2020.0016
DO - 10.1089/phage.2020.0016
M3 - Journal article
SN - 2641-6530
VL - 1
SP - 137
EP - 148
JO - PHAGE: Therapy, Applications and Research
JF - PHAGE: Therapy, Applications and Research
IS - 3
ER -