A monomer-trimer model supports intermittent glucagon fibril growth

Andrej Košmrlj; Pia Cordsen; Anders Kyrsting; Daniel Otzen; Lene B Oddershede; Mogens H Jensen

doi:10.1038/srep09005

A monomer-trimer model supports intermittent glucagon fibril growth

Andrej Košmrlj, Pia Cordsen, Anders Kyrsting, Daniel Otzen, Lene B Oddershede, Mogens H Jensen

Interdisciplinary Nanoscience Center - INANO-MBG, iNANO-huset

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

7 Citationer (Scopus)

Abstract

We investigate in vitro fibrillation kinetics of the hormone peptide glucagon at various concentrations using confocal microscopy and determine the glucagon fibril persistence length 60μm. At all concentrations we observe that periods of individual fibril growth are interrupted by periods of stasis. The growth probability is large at high and low concentrations and is reduced for intermediate glucagon concentrations. To explain this behavior we propose a simple model, where fibrils come in two forms, one built entirely from glucagon monomers and one entirely from glucagon trimers. The opposite building blocks act as fibril growth blockers, and this generic model reproduces experimental behavior well.

Originalsprog	Engelsk
Artikelnummer	9005
Tidsskrift	Scientific Reports
Vol/bind	5
Sider (fra-til)	1-6
Antal sider	6
ISSN	2045-2322
DOI	https://doi.org/10.1038/srep09005
Status	Udgivet - 11 mar. 2015

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10.1038/srep09005

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abstract = "We investigate in vitro fibrillation kinetics of the hormone peptide glucagon at various concentrations using confocal microscopy and determine the glucagon fibril persistence length 60μm. At all concentrations we observe that periods of individual fibril growth are interrupted by periods of stasis. The growth probability is large at high and low concentrations and is reduced for intermediate glucagon concentrations. To explain this behavior we propose a simple model, where fibrils come in two forms, one built entirely from glucagon monomers and one entirely from glucagon trimers. The opposite building blocks act as fibril growth blockers, and this generic model reproduces experimental behavior well.",

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AU - Košmrlj, Andrej

AU - Cordsen, Pia

AU - Kyrsting, Anders

AU - Otzen, Daniel

AU - Oddershede, Lene B

AU - Jensen, Mogens H

PY - 2015/3/11

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N2 - We investigate in vitro fibrillation kinetics of the hormone peptide glucagon at various concentrations using confocal microscopy and determine the glucagon fibril persistence length 60μm. At all concentrations we observe that periods of individual fibril growth are interrupted by periods of stasis. The growth probability is large at high and low concentrations and is reduced for intermediate glucagon concentrations. To explain this behavior we propose a simple model, where fibrils come in two forms, one built entirely from glucagon monomers and one entirely from glucagon trimers. The opposite building blocks act as fibril growth blockers, and this generic model reproduces experimental behavior well.

AB - We investigate in vitro fibrillation kinetics of the hormone peptide glucagon at various concentrations using confocal microscopy and determine the glucagon fibril persistence length 60μm. At all concentrations we observe that periods of individual fibril growth are interrupted by periods of stasis. The growth probability is large at high and low concentrations and is reduced for intermediate glucagon concentrations. To explain this behavior we propose a simple model, where fibrils come in two forms, one built entirely from glucagon monomers and one entirely from glucagon trimers. The opposite building blocks act as fibril growth blockers, and this generic model reproduces experimental behavior well.

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A monomer-trimer model supports intermittent glucagon fibril growth

Abstract

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