A Metric Fractionator for Estimation of Total Cell Number in Paraffin-Embedded Flat or Hollow Organs

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Standard

A Metric Fractionator for Estimation of Total Cell Number in Paraffin-Embedded Flat or Hollow Organs. / Mirabile, Rosanna; Boyce, Rogely Waite; Gundersen, Hans Jørgen G.

I: Toxicologic Pathology, Bind 48, Nr. 6, 08.2020, s. 784-790.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Mirabile, R, Boyce, RW & Gundersen, HJG 2020, 'A Metric Fractionator for Estimation of Total Cell Number in Paraffin-Embedded Flat or Hollow Organs', Toxicologic Pathology, bind 48, nr. 6, s. 784-790. https://doi.org/10.1177/0192623320914582

APA

Mirabile, R., Boyce, R. W., & Gundersen, H. J. G. (2020). A Metric Fractionator for Estimation of Total Cell Number in Paraffin-Embedded Flat or Hollow Organs. Toxicologic Pathology, 48(6), 784-790. https://doi.org/10.1177/0192623320914582

CBE

MLA

Mirabile, Rosanna, Rogely Waite Boyce og Hans Jørgen G. Gundersen. "A Metric Fractionator for Estimation of Total Cell Number in Paraffin-Embedded Flat or Hollow Organs". Toxicologic Pathology. 2020, 48(6). 784-790. https://doi.org/10.1177/0192623320914582

Vancouver

Author

Mirabile, Rosanna ; Boyce, Rogely Waite ; Gundersen, Hans Jørgen G. / A Metric Fractionator for Estimation of Total Cell Number in Paraffin-Embedded Flat or Hollow Organs. I: Toxicologic Pathology. 2020 ; Bind 48, Nr. 6. s. 784-790.

Bibtex

@article{ac56bb3a462d40b38f4c327f17c07095,
title = "A Metric Fractionator for Estimation of Total Cell Number in Paraffin-Embedded Flat or Hollow Organs",
abstract = "The physical fractionator is a convenient and practical solution for estimation of total cell number in a regulatory toxicology setting because it is insensitive to shrinkage allowing for paraffin processing/embedding and does not require measurement of the reference or organ volume. The principle involves sampling a known fraction of an organ in one or more steps and counting the total number of cells present in the final sample, physical disector section pairs. The total cell number in the organ is estimated by multiplying the cell count in the final fraction by the inverse of the sampling fraction(s). The key feature of the design is that tissue shrinkage due to paraffin processing occurs before the organ is uniformly sampled. Another requirement is that thermal expansion or contraction is avoided during the preparation of disector sections from the individual embedded subsamples, which ensures that the disector sections represent a known constant fraction. This vertical physical fractionator with subsampling is a simple and fast estimator to obtain precise and robust estimates of total cell number in large flat or hollow organs that do not prolong routine necropsy procedures. It is compatible with paraffin processing, avoids exhaustive sectioning, and allows for the collection of routine histopathology sections.",
keywords = "cell number, flat organ, metric fractionator, paraffin, primate, stereology, transitional epithelial cells, urinary bladder",
author = "Rosanna Mirabile and Boyce, {Rogely Waite} and Gundersen, {Hans J{\o}rgen G.}",
year = "2020",
month = aug,
doi = "10.1177/0192623320914582",
language = "English",
volume = "48",
pages = "784--790",
journal = "Toxicologic Pathology",
issn = "0192-6233",
publisher = "Sage Publications, Inc.",
number = "6",

}

RIS

TY - JOUR

T1 - A Metric Fractionator for Estimation of Total Cell Number in Paraffin-Embedded Flat or Hollow Organs

AU - Mirabile, Rosanna

AU - Boyce, Rogely Waite

AU - Gundersen, Hans Jørgen G.

PY - 2020/8

Y1 - 2020/8

N2 - The physical fractionator is a convenient and practical solution for estimation of total cell number in a regulatory toxicology setting because it is insensitive to shrinkage allowing for paraffin processing/embedding and does not require measurement of the reference or organ volume. The principle involves sampling a known fraction of an organ in one or more steps and counting the total number of cells present in the final sample, physical disector section pairs. The total cell number in the organ is estimated by multiplying the cell count in the final fraction by the inverse of the sampling fraction(s). The key feature of the design is that tissue shrinkage due to paraffin processing occurs before the organ is uniformly sampled. Another requirement is that thermal expansion or contraction is avoided during the preparation of disector sections from the individual embedded subsamples, which ensures that the disector sections represent a known constant fraction. This vertical physical fractionator with subsampling is a simple and fast estimator to obtain precise and robust estimates of total cell number in large flat or hollow organs that do not prolong routine necropsy procedures. It is compatible with paraffin processing, avoids exhaustive sectioning, and allows for the collection of routine histopathology sections.

AB - The physical fractionator is a convenient and practical solution for estimation of total cell number in a regulatory toxicology setting because it is insensitive to shrinkage allowing for paraffin processing/embedding and does not require measurement of the reference or organ volume. The principle involves sampling a known fraction of an organ in one or more steps and counting the total number of cells present in the final sample, physical disector section pairs. The total cell number in the organ is estimated by multiplying the cell count in the final fraction by the inverse of the sampling fraction(s). The key feature of the design is that tissue shrinkage due to paraffin processing occurs before the organ is uniformly sampled. Another requirement is that thermal expansion or contraction is avoided during the preparation of disector sections from the individual embedded subsamples, which ensures that the disector sections represent a known constant fraction. This vertical physical fractionator with subsampling is a simple and fast estimator to obtain precise and robust estimates of total cell number in large flat or hollow organs that do not prolong routine necropsy procedures. It is compatible with paraffin processing, avoids exhaustive sectioning, and allows for the collection of routine histopathology sections.

KW - cell number

KW - flat organ

KW - metric fractionator

KW - paraffin

KW - primate

KW - stereology

KW - transitional epithelial cells

KW - urinary bladder

UR - http://www.scopus.com/inward/record.url?scp=85084992266&partnerID=8YFLogxK

U2 - 10.1177/0192623320914582

DO - 10.1177/0192623320914582

M3 - Journal article

C2 - 32342734

AN - SCOPUS:85084992266

VL - 48

SP - 784

EP - 790

JO - Toxicologic Pathology

JF - Toxicologic Pathology

SN - 0192-6233

IS - 6

ER -