A Lotus japonicus cytoplasmic kinase connects Nod factor perception by the NFR5 LysM receptor to nodulation

Jaslyn E.M.M. Wong, Marcin Nadzieja, Lene H. Madsen, Christoph A. Bücherl, Svend Dam, Niels N. Sandal, Daniel Couto, Paul Derbyshire, Mette Uldum-Berentsen, Sina Schroeder, Veit Schwämmle, Fábio C.S. Nogueira, Mette H. Asmussen, Søren Thirup, Simona Radutoiu, Mickaël Blaise, Kasper R. Andersen, Frank L.H. Menke, Cyril Zipfel, Jens Stougaard*

*Corresponding author af dette arbejde

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Abstract

The establishment of nitrogen-fixing root nodules in legume–rhi-zobia symbiosis requires an intricate communication between the host plant and its symbiont. We are, however, limited in our understanding of the symbiosis signaling process. In particular, how membrane-localized receptors of legumes activate signal transduction following perception of rhizobial signaling molecules has mostly remained elusive. To address this, we performed a coimmunoprecipitation-based proteomics screen to identify proteins associated with Nod factor receptor 5 (NFR5) in Lotus japonicus. Out of 51 NFR5-associated proteins, we focused on a receptor-like cytoplasmic kinase (RLCK), which we named NFR5-interacting cytoplasmic kinase 4 (NiCK4). NiCK4 associates with heterologously expressed NFR5 in Nicotiana benthamiana, and directly binds and phosphorylates the cytoplasmic domains of NFR5 and NFR1 in vitro. At the cellular level, Nick4 is coexpressed with Nfr5 in root hairs and nodule cells, and the NiCK4 protein relocates to the nucleus in an NFR5/NFR1-dependent manner upon Nod factor treatment. Phenotyping of retrotransposon insertion mutants revealed that NiCK4 promotes nodule organogenesis. Together, these results suggest that the identified RLCK, NiCK4, acts as a component of the Nod factor signaling pathway downstream of NFR5.

OriginalsprogEngelsk
TidsskriftProceedings of the National Academy of Sciences
Vol/bind116
Nummer28
Sider (fra-til)14339-14348
Antal sider10
ISSN0027-8424
DOI
StatusUdgivet - 2019

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