A Heterochromatin-Specific RNA Export Pathway Facilitates piRNA Production

Mostafa F ElMaghraby, Peter Refsing Andersen, Florian Pühringer, Ulrich Hohmann, Katharina Meixner, Thomas Lendl, Laszlo Tirian, Julius Brennecke

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

59 Citationer (Scopus)

Abstract

PIWI-interacting RNAs (piRNAs) guide transposon silencing in animals. The 22–30 nt piRNAs are processed in the cytoplasm from long non-coding RNAs that often lack RNA processing hallmarks of export-competent transcripts. By studying how these transcripts achieve nuclear export, we uncover an RNA export pathway specific for piRNA precursors in the Drosophila germline. This pathway requires Nxf3-Nxt1, a variant of the hetero-dimeric mRNA export receptor Nxf1-Nxt1. Nxf3 interacts with UAP56, a nuclear RNA helicase essential for mRNA export, and CG13741/Bootlegger, which recruits Nxf3-Nxt1 and UAP56 to heterochromatic piRNA source loci. Upon RNA cargo binding, Nxf3 achieves nuclear export via the exportin Crm1 and accumulates together with Bootlegger in peri-nuclear nuage, suggesting that after export, Nxf3-Bootlegger delivers precursor transcripts to the piRNA processing sites. These findings indicate that the piRNA pathway bypasses nuclear RNA surveillance systems to export unprocessed transcripts to the cytoplasm, a strategy also exploited by retroviruses. Heterochromatic piRNA precursors escape classic mRNA quality-control and surveillance mechanisms through a dedicated nuclear export pathway.

OriginalsprogEngelsk
TidsskriftCell
Vol/bind178
Nummer4
Sider (fra-til)964-979
ISSN0092-8674
DOI
StatusUdgivet - 8 aug. 2019
Udgivet eksterntJa

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