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A five-transgene cassette confers broad-spectrum resistance to a fungal rust pathogen in wheat

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DOI

  • Ming Luo, CSIRO Agriculture and Food, Australien
  • Liqiong Xie, Xinjiang University, Kina
  • Soma Chakraborty, CSIRO Agriculture and Food, Australien
  • Aihua Wang, CSIRO Agriculture and Food, Kina
  • Oadi Matny, University of Minnesota, St. Paul, USA
  • Michelle Jugovich, University of Minnesota, St. Paul, USA
  • James A Kolmer, USDA-ARS, Cereal Disease Laboratory, USA
  • Terese Richardson, CSIRO Agriculture and Food, Australien
  • Dhara Bhatt, CSIRO Agriculture and Food, Australien
  • Mohammad Hoque, CSIRO Agriculture and Food, Australien
  • Mehran Patpour
  • Chris Sørensen
  • Diana Ortiz, National Research Institute for Agriculture, Food and Environment, Frankrig
  • Peter Dodds, CSIRO Agriculture and Food, Australien
  • Burkhard Steuernagel, John Innes Centre, Norwich, Storbritannien
  • Brande B H Wulff, John Innes Centre, Norwich, Storbritannien
  • Narayana M Upadhyaya, CSIRO Agriculture and Food, Australien
  • Rohit Mago, CSIRO Agriculture and Food, Australien
  • Sambasivam Periyannan, CSIRO Agriculture and Food, Australien
  • Evans Lagudah, CSIRO Agriculture and Food, Australien
  • Roger Freedman, 2Blades Foundation, Evanston, USA
  • T Lynne Reuber, 2Blades Foundation, Evanston, USA
  • Brian J Steffenson, University of Minnesota, St. Paul, USA
  • Michael Ayliffe, CSIRO Agriculture and Food, Australien

Breeding wheat with durable resistance to the fungal pathogen Puccinia graminis f. sp. tritici (Pgt), a major threat to cereal production, is challenging due to the rapid evolution of pathogen virulence. Increased durability and broad-spectrum resistance can be achieved by introducing more than one resistance gene, but combining numerous unlinked genes by breeding is laborious. Here we generate polygenic Pgt resistance by introducing a transgene cassette of five resistance genes into bread wheat as a single locus and show that at least four of the five genes are functional. These wheat lines are resistant to aggressive and highly virulent Pgt isolates from around the world and show very high levels of resistance in the field. The simple monogenic inheritance of this multigene locus greatly simplifies its use in breeding. However, a new Pgt isolate with virulence to several genes at this locus suggests gene stacks will need strategic deployment to maintain their effectiveness.

OriginalsprogEngelsk
TidsskriftNature Biotechnology
Vol/bind39
Nummer5
Sider (fra-til)561-566
Antal sider6
ISSN1087-0156
DOI
StatusUdgivet - maj 2021

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