A DNA sequence obtained by replacement of the dopamine RNA aptamer bases is not an aptamer

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

A DNA sequence obtained by replacement of the dopamine RNA aptamer bases is not an aptamer. / Álvarez-Martos, Isabel; Ferapontova, Elena.

I: Biochemical and Biophysical Research Communications, Bind 489, Nr. 4, 05.08.2017, s. 381-385.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Álvarez-Martos, I & Ferapontova, E 2017, 'A DNA sequence obtained by replacement of the dopamine RNA aptamer bases is not an aptamer', Biochemical and Biophysical Research Communications, bind 489, nr. 4, s. 381-385. https://doi.org/10.1016/j.bbrc.2017.05.134

APA

CBE

MLA

Vancouver

Álvarez-Martos I, Ferapontova E. A DNA sequence obtained by replacement of the dopamine RNA aptamer bases is not an aptamer. Biochemical and Biophysical Research Communications. 2017 aug 5;489(4):381-385. https://doi.org/10.1016/j.bbrc.2017.05.134

Author

Álvarez-Martos, Isabel ; Ferapontova, Elena. / A DNA sequence obtained by replacement of the dopamine RNA aptamer bases is not an aptamer. I: Biochemical and Biophysical Research Communications. 2017 ; Bind 489, Nr. 4. s. 381-385.

Bibtex

@article{f3a5bc2912cc4d69bfc32bc21029b127,
title = "A DNA sequence obtained by replacement of the dopamine RNA aptamer bases is not an aptamer",
abstract = "A unique specificity of the aptamer-ligand biorecognition and binding facilitates bioanalysis and biosensor development, contributing to discrimination of structurally related molecules, such as dopamine and other catecholamine neurotransmitters. The aptamer sequence capable of specific binding of dopamine is a 57 nucleotides long RNA sequence reported in 1997 (Biochemistry, 1997, 36, 9726). Later, it was suggested that the DNA homologue of the RNA aptamer retains the specificity of dopamine binding (Biochem. Biophys. Res. Commun., 2009, 388, 732). Here, we show that the DNA sequence obtained by the replacement of the RNA aptamer bases for their DNA analogues is not able of specific biorecognition of dopamine, in contrast to the original RNA aptamer sequence. This DNA sequence binds dopamine and structurally related catecholamine neurotransmitters non-specifically, as any DNA sequence, and, thus, is not an aptamer and cannot be used neither for in vivo nor in situ analysis of dopamine in the presence of structurally related neurotransmitters.",
keywords = "Dopamine, Electrochemistry, RNA APTAMER, DNA",
author = "Isabel {\'A}lvarez-Martos and Elena Ferapontova",
year = "2017",
month = aug,
day = "5",
doi = "10.1016/j.bbrc.2017.05.134",
language = "English",
volume = "489",
pages = "381--385",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier Inc.",
number = "4",

}

RIS

TY - JOUR

T1 - A DNA sequence obtained by replacement of the dopamine RNA aptamer bases is not an aptamer

AU - Álvarez-Martos, Isabel

AU - Ferapontova, Elena

PY - 2017/8/5

Y1 - 2017/8/5

N2 - A unique specificity of the aptamer-ligand biorecognition and binding facilitates bioanalysis and biosensor development, contributing to discrimination of structurally related molecules, such as dopamine and other catecholamine neurotransmitters. The aptamer sequence capable of specific binding of dopamine is a 57 nucleotides long RNA sequence reported in 1997 (Biochemistry, 1997, 36, 9726). Later, it was suggested that the DNA homologue of the RNA aptamer retains the specificity of dopamine binding (Biochem. Biophys. Res. Commun., 2009, 388, 732). Here, we show that the DNA sequence obtained by the replacement of the RNA aptamer bases for their DNA analogues is not able of specific biorecognition of dopamine, in contrast to the original RNA aptamer sequence. This DNA sequence binds dopamine and structurally related catecholamine neurotransmitters non-specifically, as any DNA sequence, and, thus, is not an aptamer and cannot be used neither for in vivo nor in situ analysis of dopamine in the presence of structurally related neurotransmitters.

AB - A unique specificity of the aptamer-ligand biorecognition and binding facilitates bioanalysis and biosensor development, contributing to discrimination of structurally related molecules, such as dopamine and other catecholamine neurotransmitters. The aptamer sequence capable of specific binding of dopamine is a 57 nucleotides long RNA sequence reported in 1997 (Biochemistry, 1997, 36, 9726). Later, it was suggested that the DNA homologue of the RNA aptamer retains the specificity of dopamine binding (Biochem. Biophys. Res. Commun., 2009, 388, 732). Here, we show that the DNA sequence obtained by the replacement of the RNA aptamer bases for their DNA analogues is not able of specific biorecognition of dopamine, in contrast to the original RNA aptamer sequence. This DNA sequence binds dopamine and structurally related catecholamine neurotransmitters non-specifically, as any DNA sequence, and, thus, is not an aptamer and cannot be used neither for in vivo nor in situ analysis of dopamine in the presence of structurally related neurotransmitters.

KW - Dopamine

KW - Electrochemistry

KW - RNA APTAMER

KW - DNA

U2 - 10.1016/j.bbrc.2017.05.134

DO - 10.1016/j.bbrc.2017.05.134

M3 - Journal article

C2 - 28576492

VL - 489

SP - 381

EP - 385

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -