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A Deep Eutectic Solvent Thermomorphic Multiphasic System for Biocatalytic Applications

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The applicability of a thermomorphic multiphasic system (TMS) composed of a hydrophobic deep eutectic solvent (DES) and an aqueous potassium phosphate buffer with a lower critical solution temperature (LCST) phase change for homogeneous biocatalysis was investigated. A lidocaine-based DES with fatty acid oleic acid as hydrogen bond donor was studied. Phase diagrams were determined and presented within this study. We tested different additional components to the solvent system and observed a decrease of the cloud point of ca. 0.026 °C per concentration unit. Distribution studies were performed revealing a clear distribution of the protein in the aqueous buffer phase (>95%) whereas the hydrophobic substrate and educt accumulate >95% in the DES-enriched layer. Finally, a horse liver alcohol dehydrogenase-catalyzed reduction was performed in a larger scale experiment and the biocatalyst could be recycled by simply removing the DES phase for three recycling runs.
OriginalsprogEngelsk
Artikelnummere202203823
TidsskriftAngewandte Chemie International Edition
Vol/bind61
Nummer31
Antal sider8
ISSN1433-7851
DOI
StatusUdgivet - 1 aug. 2022

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© 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.

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