A Deep Eutectic Solvent Thermomorphic Multiphasic System for Biocatalytic Applications

Lars-Erik Meyer*, Mads Bruno Andersen, Selin Kara*

*Corresponding author af dette arbejde

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The applicability of a thermomorphic multiphasic system (TMS) composed of a hydrophobic deep eutectic solvent (DES) and an aqueous potassium phosphate buffer with a lower critical solution temperature (LCST) phase change for homogeneous biocatalysis was investigated. A lidocaine-based DES with fatty acid oleic acid as hydrogen bond donor was studied. Phase diagrams were determined and presented within this study. We tested different additional components to the solvent system and observed a decrease of the cloud point of ca. 0.026 °C per concentration unit. Distribution studies were performed revealing a clear distribution of the protein in the aqueous buffer phase (>95%) whereas the hydrophobic substrate and educt accumulate >95% in the DES-enriched layer. Finally, a horse liver alcohol dehydrogenase-catalyzed reduction was performed in a larger scale experiment and the biocatalyst could be recycled by simply removing the DES phase for three recycling runs.
TidsskriftAngewandte Chemie International Edition
Antal sider8
StatusUdgivet - 1 aug. 2022


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