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A confocal microscopy based method to monitor extracellular pH in fungal biofilms

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Standard

A confocal microscopy based method to monitor extracellular pH in fungal biofilms. / Schlafer, Sebastian; Kamp, Anja; Garcia, Javier.

I: FEMS Yeast Research, Bind 18, Nr. 5, 049, 2018.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Harvard

Schlafer, S, Kamp, A & Garcia, J 2018, 'A confocal microscopy based method to monitor extracellular pH in fungal biofilms', FEMS Yeast Research, bind 18, nr. 5, 049. https://doi.org/10.1093/femsyr/foy049

APA

Schlafer, S., Kamp, A., & Garcia, J. (2018). A confocal microscopy based method to monitor extracellular pH in fungal biofilms. FEMS Yeast Research, 18(5), [049]. https://doi.org/10.1093/femsyr/foy049

CBE

Schlafer S, Kamp A, Garcia J. 2018. A confocal microscopy based method to monitor extracellular pH in fungal biofilms. FEMS Yeast Research. 18(5):Article 049. https://doi.org/10.1093/femsyr/foy049

MLA

Schlafer, Sebastian, Anja Kamp, og Javier Garcia. "A confocal microscopy based method to monitor extracellular pH in fungal biofilms". FEMS Yeast Research. 2018. 18(5). https://doi.org/10.1093/femsyr/foy049

Vancouver

Schlafer S, Kamp A, Garcia J. A confocal microscopy based method to monitor extracellular pH in fungal biofilms. FEMS Yeast Research. 2018;18(5):049. doi: 10.1093/femsyr/foy049

Author

Schlafer, Sebastian ; Kamp, Anja ; Garcia, Javier. / A confocal microscopy based method to monitor extracellular pH in fungal biofilms. I: FEMS Yeast Research. 2018 ; Bind 18, Nr. 5.

Bibtex

@article{d2836539c6784ea8a22f87894bce5def,
title = "A confocal microscopy based method to monitor extracellular pH in fungal biofilms",
abstract = "pH in fungal biofilms is important for a variety of fungal infections and industrial applications involving fungal biofilms, but to date, it has never been measured directly inside the biofilms matrix. In the present study, a new methodology was developed allowing for confocal microscopy based monitoring of extracellular pH inside fungal biofilms. Monospecies biofilms of Aspergillus fumigatus, Candida albicans, Candida dubliniensis and Cryptococcus neoformans were stained with the pH dependent ratiometric probe C-SNARF-4, imaged with a confocal microscope, and a digital image analysis procedure was developed to determine pH in the extracellular matrix. As a proof of concept, pH developments at the biofilms–substratum interface were monitored for 1 h after exposure to glucose. Observed pH drops differed considerably between the different species and also between replicate biofilms of the same species. Candida albicans biofilms showed the highest acidogenicity, with pH drops occurring much faster than in planktonic culture. pH ratiometry with C-SNARF-4 is a valuable tool to get insight into fungal biofilm metabolism and may shed new light on both disease-related and industrially relevant processes in fungal biofilms.",
keywords = "Aspergillus, Candida, confocal laser scanning microscopy, Cryptococcus, C-SNARF-4, Saccharomyces",
author = "Sebastian Schlafer and Anja Kamp and Javier Garcia",
year = "2018",
doi = "10.1093/femsyr/foy049",
language = "English",
volume = "18",
journal = "FEMS Yeast Research",
issn = "1567-1356",
publisher = "Oxford University Press",
number = "5",

}

RIS

TY - JOUR

T1 - A confocal microscopy based method to monitor extracellular pH in fungal biofilms

AU - Schlafer, Sebastian

AU - Kamp, Anja

AU - Garcia, Javier

PY - 2018

Y1 - 2018

N2 - pH in fungal biofilms is important for a variety of fungal infections and industrial applications involving fungal biofilms, but to date, it has never been measured directly inside the biofilms matrix. In the present study, a new methodology was developed allowing for confocal microscopy based monitoring of extracellular pH inside fungal biofilms. Monospecies biofilms of Aspergillus fumigatus, Candida albicans, Candida dubliniensis and Cryptococcus neoformans were stained with the pH dependent ratiometric probe C-SNARF-4, imaged with a confocal microscope, and a digital image analysis procedure was developed to determine pH in the extracellular matrix. As a proof of concept, pH developments at the biofilms–substratum interface were monitored for 1 h after exposure to glucose. Observed pH drops differed considerably between the different species and also between replicate biofilms of the same species. Candida albicans biofilms showed the highest acidogenicity, with pH drops occurring much faster than in planktonic culture. pH ratiometry with C-SNARF-4 is a valuable tool to get insight into fungal biofilm metabolism and may shed new light on both disease-related and industrially relevant processes in fungal biofilms.

AB - pH in fungal biofilms is important for a variety of fungal infections and industrial applications involving fungal biofilms, but to date, it has never been measured directly inside the biofilms matrix. In the present study, a new methodology was developed allowing for confocal microscopy based monitoring of extracellular pH inside fungal biofilms. Monospecies biofilms of Aspergillus fumigatus, Candida albicans, Candida dubliniensis and Cryptococcus neoformans were stained with the pH dependent ratiometric probe C-SNARF-4, imaged with a confocal microscope, and a digital image analysis procedure was developed to determine pH in the extracellular matrix. As a proof of concept, pH developments at the biofilms–substratum interface were monitored for 1 h after exposure to glucose. Observed pH drops differed considerably between the different species and also between replicate biofilms of the same species. Candida albicans biofilms showed the highest acidogenicity, with pH drops occurring much faster than in planktonic culture. pH ratiometry with C-SNARF-4 is a valuable tool to get insight into fungal biofilm metabolism and may shed new light on both disease-related and industrially relevant processes in fungal biofilms.

KW - Aspergillus

KW - Candida

KW - confocal laser scanning microscopy

KW - Cryptococcus

KW - C-SNARF-4

KW - Saccharomyces

UR - http://www.scopus.com/inward/record.url?scp=85050638528&partnerID=8YFLogxK

U2 - 10.1093/femsyr/foy049

DO - 10.1093/femsyr/foy049

M3 - Journal article

C2 - 29684195

VL - 18

JO - FEMS Yeast Research

JF - FEMS Yeast Research

SN - 1567-1356

IS - 5

M1 - 049

ER -