A comparison of GenomEra® GBS PCR and GeneXpert ® GBS PCR assays with culture of GBS performed with and without broth pre-enrichment

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A comparison of GenomEra® GBS PCR and GeneXpert ® GBS PCR assays with culture of GBS performed with and without broth pre-enrichment. / Nielsen, S. Y.; Møller, J. K.; Khalil, M. R.

I: European Journal of Clinical Microbiology and Infectious Diseases, Bind 39, Nr. 10, 2020, s. 1945-1950.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Nielsen, SY, Møller, JK & Khalil, MR 2020, 'A comparison of GenomEra® GBS PCR and GeneXpert ® GBS PCR assays with culture of GBS performed with and without broth pre-enrichment', European Journal of Clinical Microbiology and Infectious Diseases, bind 39, nr. 10, s. 1945-1950. https://doi.org/10.1007/s10096-020-03934-4

APA

Nielsen, S. Y., Møller, J. K., & Khalil, M. R. (2020). A comparison of GenomEra® GBS PCR and GeneXpert ® GBS PCR assays with culture of GBS performed with and without broth pre-enrichment. European Journal of Clinical Microbiology and Infectious Diseases, 39(10), 1945-1950. https://doi.org/10.1007/s10096-020-03934-4

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MLA

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Author

Nielsen, S. Y. ; Møller, J. K. ; Khalil, M. R. / A comparison of GenomEra® GBS PCR and GeneXpert ® GBS PCR assays with culture of GBS performed with and without broth pre-enrichment. I: European Journal of Clinical Microbiology and Infectious Diseases. 2020 ; Bind 39, Nr. 10. s. 1945-1950.

Bibtex

@article{005eb1aa033f454c80b5681eeb842cf6,
title = "A comparison of GenomEra{\textregistered} GBS PCR and GeneXpert {\textregistered} GBS PCR assays with culture of GBS performed with and without broth pre-enrichment",
abstract = "This study was designed to compare the performance of GeneXpert{\textregistered} and GenomEra{\textregistered} group B streptococcus (GBS) PCR assays, held up against standard culture of GBS performed with and without broth pre-enrichment. In Denmark, the strategy for preventing early onset GBS infection (EOGBS) is risk factor based. Three hundred and sixty six women fulfilling one or more of the criteria for presence of risk factors for EOGBS were prospectively included. Rectovaginal swab samples were taken intrapartum and tested bed-site by the GenomEra{\textregistered} and the GeneXpert{\textregistered} GBS PCR assays and cultured at the microbiology laboratory using Granada agar plates with and without prior growth of sampling material in selective enrichment broth. Among 366 participants tested intrapartum, 99 were GBS-positive by culture, 95 by GenomEra, and 95 by GeneXpert. Compared with culture, the GenomEra and the GeneXpert performed with a sensitivity of 91.8% and 91.7% and a specificity of 98.1% and 97.3%, respectively. A combined reference standard was established by defining true positives as either culture-positive samples or culture-negative samples where both the GeneXpert and the GenomEra GBS PCR assays were positive. Using this, the sensitivity increased to 92.2% and the specificity to 99.6% for GenomEra and to 92.0% and 96.8% for GeneXpert. The use of selective broth enrichment found only three additional GBS culture-positive samples. The performance of the two PCR methods examined was very similar and close to the findings by culture, and both PCR assays are thus applicable as rapid intrapartum bed-site tests.",
keywords = "Early onset, EOGBS, GBS, Group B streptococci, Intrapartum, PCR, Point-of-care testing, Pregnancy, S. agalactiae",
author = "Nielsen, {S. Y.} and M{\o}ller, {J. K.} and Khalil, {M. R.}",
year = "2020",
doi = "10.1007/s10096-020-03934-4",
language = "English",
volume = "39",
pages = "1945--1950",
journal = "European Journal of Clinical Microbiology & Infectious Diseases",
issn = "0934-9723",
publisher = "Springer",
number = "10",

}

RIS

TY - JOUR

T1 - A comparison of GenomEra® GBS PCR and GeneXpert ® GBS PCR assays with culture of GBS performed with and without broth pre-enrichment

AU - Nielsen, S. Y.

AU - Møller, J. K.

AU - Khalil, M. R.

PY - 2020

Y1 - 2020

N2 - This study was designed to compare the performance of GeneXpert® and GenomEra® group B streptococcus (GBS) PCR assays, held up against standard culture of GBS performed with and without broth pre-enrichment. In Denmark, the strategy for preventing early onset GBS infection (EOGBS) is risk factor based. Three hundred and sixty six women fulfilling one or more of the criteria for presence of risk factors for EOGBS were prospectively included. Rectovaginal swab samples were taken intrapartum and tested bed-site by the GenomEra® and the GeneXpert® GBS PCR assays and cultured at the microbiology laboratory using Granada agar plates with and without prior growth of sampling material in selective enrichment broth. Among 366 participants tested intrapartum, 99 were GBS-positive by culture, 95 by GenomEra, and 95 by GeneXpert. Compared with culture, the GenomEra and the GeneXpert performed with a sensitivity of 91.8% and 91.7% and a specificity of 98.1% and 97.3%, respectively. A combined reference standard was established by defining true positives as either culture-positive samples or culture-negative samples where both the GeneXpert and the GenomEra GBS PCR assays were positive. Using this, the sensitivity increased to 92.2% and the specificity to 99.6% for GenomEra and to 92.0% and 96.8% for GeneXpert. The use of selective broth enrichment found only three additional GBS culture-positive samples. The performance of the two PCR methods examined was very similar and close to the findings by culture, and both PCR assays are thus applicable as rapid intrapartum bed-site tests.

AB - This study was designed to compare the performance of GeneXpert® and GenomEra® group B streptococcus (GBS) PCR assays, held up against standard culture of GBS performed with and without broth pre-enrichment. In Denmark, the strategy for preventing early onset GBS infection (EOGBS) is risk factor based. Three hundred and sixty six women fulfilling one or more of the criteria for presence of risk factors for EOGBS were prospectively included. Rectovaginal swab samples were taken intrapartum and tested bed-site by the GenomEra® and the GeneXpert® GBS PCR assays and cultured at the microbiology laboratory using Granada agar plates with and without prior growth of sampling material in selective enrichment broth. Among 366 participants tested intrapartum, 99 were GBS-positive by culture, 95 by GenomEra, and 95 by GeneXpert. Compared with culture, the GenomEra and the GeneXpert performed with a sensitivity of 91.8% and 91.7% and a specificity of 98.1% and 97.3%, respectively. A combined reference standard was established by defining true positives as either culture-positive samples or culture-negative samples where both the GeneXpert and the GenomEra GBS PCR assays were positive. Using this, the sensitivity increased to 92.2% and the specificity to 99.6% for GenomEra and to 92.0% and 96.8% for GeneXpert. The use of selective broth enrichment found only three additional GBS culture-positive samples. The performance of the two PCR methods examined was very similar and close to the findings by culture, and both PCR assays are thus applicable as rapid intrapartum bed-site tests.

KW - Early onset

KW - EOGBS

KW - GBS

KW - Group B streptococci

KW - Intrapartum

KW - PCR

KW - Point-of-care testing

KW - Pregnancy

KW - S. agalactiae

UR - http://www.scopus.com/inward/record.url?scp=85086388964&partnerID=8YFLogxK

U2 - 10.1007/s10096-020-03934-4

DO - 10.1007/s10096-020-03934-4

M3 - Journal article

C2 - 32535806

AN - SCOPUS:85086388964

VL - 39

SP - 1945

EP - 1950

JO - European Journal of Clinical Microbiology & Infectious Diseases

JF - European Journal of Clinical Microbiology & Infectious Diseases

SN - 0934-9723

IS - 10

ER -