A broad-host range dual-fluorescence reporter system for gene expression analysis in Gram-negative bacteria

Rosanna C. Hennessy, Line Christiansen, Stefan Olsson, Peter Stougaard

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Abstract

Fluorescence-based reporter systems are valuable tools for studying gene expression dynamics in living cells. Here we describe a dual-fluorescence reporter system carrying the red fluorescent marker mCherry and the blue fluorescent protein EBFP2 enabling the simultaneous analysis of two promoters in broad-host range autofluorescent Gram-negative bacteria.

OriginalsprogEngelsk
TidsskriftJournal of Microbiological Methods
Vol/bind144
Sider (fra-til)173-176
Antal sider4
ISSN0167-7012
StatusUdgivet - 2017
Udgivet eksterntJa

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