[99mTc]-labelled Interleukin-8 as a Diagnostic Tool Compared to [18F]FDG and CT in an Experimental Porcine Osteomyelitis Model

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  • Pia Afzelius, North Zealand Hospital, Aalborg Universitet, Danmark
  • Peter M. H. Heegaard, Danish Technical University, Danmark
  • Svend Borup Jensen, Aalborg Universitet, Danmark
  • Aage Kristian Olsen Alstrup
  • Henrik Carl Schønheyder
  • Annemarie Eek, Raboud University Nijmegen.
  • ,
  • Otto Boerman, Raboud University Nijmegen.
  • ,
  • Ole Lerberg Nielsen, Københavns Universitet, Danmark
Background: Osteomyelitis (OM) is an important cause of morbidity and sometimes even mortality in children and adults. Long-term complications can be reduced when treatment is initiated in an early phase of the infection. The diagnostic gold standard is microbial examination of a biopsy and current non-invasive imaging methods are not optimal in all cases. [111In]-leukocyte scintigraphy is recommended for peripheral OM, but is timeconsuming
and not recommended in children. [18F]FDG PET/CT is recommended for
vertebral OM in adults, but has the disadvantage of false positive findings and a relatively high radiation exposure; the latter is a problem especially in children. [99mTc]-based tracers are consequently preferred in children. We, therefore, aimed to find a [99mTc]-marked tracer with high specificity and sensitivity for early detection of OM. Suppurating inflammatory lesions like OM caused by Staphylococcus aureus (S. aureus) will attract large numbers of neutrophils and macrophages. A preliminary study has shown that [99mTc]-labelled IL8 may
be a possible candidate for imaging of peripheral OM.
Methods: We investigated [99mTc]IL8 scintigraphy in a juvenile pig model of peripheral OM and compared it with [18F]FDG PET/CT. The pigs were experimentally inoculated with S.aureus to induce OM and scanned one week later. We also examined leukocyte count, serum CRP and IL8, as well as performed histopathological and microbiological investigations.
Results: [99mTc]IL8 was easily and relatively quickly prepared and was shown to be suitable for visualization of OM lesions in peripheral bones of juvenile pigs detecting 70% compared to a 100% sensitivity of [18F]FDG PET/CT.
Conclusion: [99mTc]IL8 is a promising candidate for detection of OM in peripheral bones in children.
OriginalsprogEngelsk
TidsskriftAmerican Journal of Nuclear Medicine and Molecular Imaging
Vol/bind10
Nummer1
Sider (fra-til)32-46
Antal sider15
ISSN2160-8407
StatusUdgivet - 2020

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