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3' End sequencing of pA+ and pA- RNAs

Publikation: Bidrag til bog/antologi/rapport/proceedingBidrag til bog/antologiForskning

Standard

3' End sequencing of pA+ and pA- RNAs. / Wu, Guifen; Schmid, Manfred; Jensen, Torben Heick.

mRNA 3' End Processing and Metabolism. red. / Bin Tian. Cambridge, MA : Academic Press, 2021. s. 139-164 (Methods in Enzymology, Bind 655).

Publikation: Bidrag til bog/antologi/rapport/proceedingBidrag til bog/antologiForskning

Harvard

Wu, G, Schmid, M & Jensen, TH 2021, 3' End sequencing of pA+ and pA- RNAs. i B Tian (red.), mRNA 3' End Processing and Metabolism. Academic Press, Cambridge, MA, Methods in Enzymology, bind 655, s. 139-164. https://doi.org/10.1016/bs.mie.2021.03.020

APA

Wu, G., Schmid, M., & Jensen, T. H. (2021). 3' End sequencing of pA+ and pA- RNAs. I B. Tian (red.), mRNA 3' End Processing and Metabolism (s. 139-164). Academic Press. Methods in Enzymology Bind 655 https://doi.org/10.1016/bs.mie.2021.03.020

CBE

Wu G, Schmid M, Jensen TH. 2021. 3' End sequencing of pA+ and pA- RNAs. Tian B, red. I mRNA 3' End Processing and Metabolism. Cambridge, MA: Academic Press. s. 139-164. (Methods in Enzymology, Bind 655). https://doi.org/10.1016/bs.mie.2021.03.020

MLA

Wu, Guifen, Manfred Schmid, og Torben Heick Jensen "3' End sequencing of pA+ and pA- RNAs". Tian, Bin (redaktører). mRNA 3' End Processing and Metabolism. Kapitel 7, Cambridge, MA: Academic Press. (Methods in Enzymology, Bind 655). 2021, 139-164. https://doi.org/10.1016/bs.mie.2021.03.020

Vancouver

Wu G, Schmid M, Jensen TH. 3' End sequencing of pA+ and pA- RNAs. I Tian B, red., mRNA 3' End Processing and Metabolism. Cambridge, MA: Academic Press. 2021. s. 139-164. (Methods in Enzymology, Bind 655). https://doi.org/10.1016/bs.mie.2021.03.020

Author

Wu, Guifen ; Schmid, Manfred ; Jensen, Torben Heick. / 3' End sequencing of pA+ and pA- RNAs. mRNA 3' End Processing and Metabolism. red. / Bin Tian. Cambridge, MA : Academic Press, 2021. s. 139-164 (Methods in Enzymology, Bind 655).

Bibtex

@inbook{51d30bd8e9484c6db9a6ee8789a91646,
title = "3' End sequencing of pA+ and pA- RNAs",
abstract = "The identity and metabolism of RNAs are often governed by their 5' and 3' ends. Single gene loci produce a variety of transcript isoforms, varying primarily in their RNA 3' end status and consequently facing radically different cellular fates. Knowledge about RNA termini is therefore key to understanding the diverse RNA output from individual transcription units. In addition, the 3' end of a nascent RNA at the catalytic center of RNA polymerase provides a precise and strand-specific measure of the transcription process. Here, we describe a modified RNA 3' end sequencing method, that utilizes the in vivo metabolic labeling of RNA followed by its purification and optional in vitro polyadenylation to provide a comprehensive view of all RNA 3' ends. The strategy offers the advantages of (i) nucleotide resolution mapping of RNA 3' ends, (ii) increased sequencing depth of lowly abundant RNA and (iii) inference of RNA 3' end polyadenylation status. We have used the method to study RNA decay and transcription termination mechanisms with the potential utility to a wider range of biological questions.",
keywords = "Polyadenylation, RNA/genetics, RNA Stability, Transcription, Genetic",
author = "Guifen Wu and Manfred Schmid and Jensen, {Torben Heick}",
note = "Copyright {\textcopyright} 2021 Elsevier Inc. All rights reserved.",
year = "2021",
doi = "10.1016/bs.mie.2021.03.020",
language = "English",
isbn = "9780128235737",
series = "Methods in Enzymology",
publisher = "Academic Press",
pages = "139--164",
editor = "Bin Tian",
booktitle = "mRNA 3' End Processing and Metabolism",

}

RIS

TY - CHAP

T1 - 3' End sequencing of pA+ and pA- RNAs

AU - Wu, Guifen

AU - Schmid, Manfred

AU - Jensen, Torben Heick

N1 - Copyright © 2021 Elsevier Inc. All rights reserved.

PY - 2021

Y1 - 2021

N2 - The identity and metabolism of RNAs are often governed by their 5' and 3' ends. Single gene loci produce a variety of transcript isoforms, varying primarily in their RNA 3' end status and consequently facing radically different cellular fates. Knowledge about RNA termini is therefore key to understanding the diverse RNA output from individual transcription units. In addition, the 3' end of a nascent RNA at the catalytic center of RNA polymerase provides a precise and strand-specific measure of the transcription process. Here, we describe a modified RNA 3' end sequencing method, that utilizes the in vivo metabolic labeling of RNA followed by its purification and optional in vitro polyadenylation to provide a comprehensive view of all RNA 3' ends. The strategy offers the advantages of (i) nucleotide resolution mapping of RNA 3' ends, (ii) increased sequencing depth of lowly abundant RNA and (iii) inference of RNA 3' end polyadenylation status. We have used the method to study RNA decay and transcription termination mechanisms with the potential utility to a wider range of biological questions.

AB - The identity and metabolism of RNAs are often governed by their 5' and 3' ends. Single gene loci produce a variety of transcript isoforms, varying primarily in their RNA 3' end status and consequently facing radically different cellular fates. Knowledge about RNA termini is therefore key to understanding the diverse RNA output from individual transcription units. In addition, the 3' end of a nascent RNA at the catalytic center of RNA polymerase provides a precise and strand-specific measure of the transcription process. Here, we describe a modified RNA 3' end sequencing method, that utilizes the in vivo metabolic labeling of RNA followed by its purification and optional in vitro polyadenylation to provide a comprehensive view of all RNA 3' ends. The strategy offers the advantages of (i) nucleotide resolution mapping of RNA 3' ends, (ii) increased sequencing depth of lowly abundant RNA and (iii) inference of RNA 3' end polyadenylation status. We have used the method to study RNA decay and transcription termination mechanisms with the potential utility to a wider range of biological questions.

KW - Polyadenylation

KW - RNA/genetics

KW - RNA Stability

KW - Transcription, Genetic

U2 - 10.1016/bs.mie.2021.03.020

DO - 10.1016/bs.mie.2021.03.020

M3 - Book chapter

C2 - 34183119

SN - 9780128235737

T3 - Methods in Enzymology

SP - 139

EP - 164

BT - mRNA 3' End Processing and Metabolism

A2 - Tian, Bin

PB - Academic Press

CY - Cambridge, MA

ER -