In situ identification of streptococci and other bacteria in initial dental biofilm by confocal laser scanning microscopy and fluorescence in situ hybridization

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  In situ identification of streptococci and other bacteria in initial dental biofilm by confocal laser scanning microscopy and fluorescence in situ hybridization. / Dige, Irene; Kilian, Mogens; Nilsson, Holger et al.

I: European Journal of Oral Sciences, Bind 115, 2007, s. 459-467.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{4831c8b08d2911dcbee902004c4f4f50,
title = "  In situ identification of streptococci and other bacteria in initial dental biofilm by confocal laser scanning microscopy and fluorescence in situ hybridization",
abstract = "Confocal laser scanning microscopy (CLSM) has been employed as a method forstudying intact natural biofilm. When combined with fluorescence in situ hybridization(FISH) it is possible to analyze spatial relationships and changes of specific membersof microbial populations over time. The aim of this study was to perform a systematicdescription of the pattern of initial dental biofilm formation by applying 16S rRNA-targeted oligonucleotide probes to the identification of streptococci and other bacteria,and to evaluate the usefulness of the combination of CLSM and FISH for structuralstudies of bacterial populations in dental biofilm. Biofilms were collected on stan-dardized glass slabs mounted in intra-oral appliances and worn by 10 individuals for 6,12, 24 or 48 h. After intra-oral exposure the biofilms were labelled with probes againsteither streptococci (STR405) or all bacteria (EUB338) and analysed by CLSM. Thecurrent approach of using FISH techniques enabled differentiation of streptococcifrom other bacteria and determination of their spatio-temporal organization. Thepresence of chimney-like multilayered microcolonies with different microbial compo-sitions demonstrated by this methodology provided information supplementary to ourprevious knowledge obtained by classical electron microscopic methods and increasedour understanding of the structure of developing biofilms.",
keywords = "biofilm, confocal laser scanning, dental plaque;, fluorescence in situ, ultrastructure",
author = "Irene Dige and Mogens Kilian and Holger Nilsson and Bente Nyvad",
year = "2007",
language = "English",
volume = "115",
pages = "459--467",
journal = "European Journal of Oral Sciences",
issn = "0909-8836",
publisher = "Wiley-Blackwell Publishing, Inc.",

}

RIS

TY - JOUR

T1 -   In situ identification of streptococci and other bacteria in initial dental biofilm by confocal laser scanning microscopy and fluorescence in situ hybridization

AU - Dige, Irene

AU - Kilian, Mogens

AU - Nilsson, Holger

AU - Nyvad, Bente

PY - 2007

Y1 - 2007

N2 - Confocal laser scanning microscopy (CLSM) has been employed as a method forstudying intact natural biofilm. When combined with fluorescence in situ hybridization(FISH) it is possible to analyze spatial relationships and changes of specific membersof microbial populations over time. The aim of this study was to perform a systematicdescription of the pattern of initial dental biofilm formation by applying 16S rRNA-targeted oligonucleotide probes to the identification of streptococci and other bacteria,and to evaluate the usefulness of the combination of CLSM and FISH for structuralstudies of bacterial populations in dental biofilm. Biofilms were collected on stan-dardized glass slabs mounted in intra-oral appliances and worn by 10 individuals for 6,12, 24 or 48 h. After intra-oral exposure the biofilms were labelled with probes againsteither streptococci (STR405) or all bacteria (EUB338) and analysed by CLSM. Thecurrent approach of using FISH techniques enabled differentiation of streptococcifrom other bacteria and determination of their spatio-temporal organization. Thepresence of chimney-like multilayered microcolonies with different microbial compo-sitions demonstrated by this methodology provided information supplementary to ourprevious knowledge obtained by classical electron microscopic methods and increasedour understanding of the structure of developing biofilms.

AB - Confocal laser scanning microscopy (CLSM) has been employed as a method forstudying intact natural biofilm. When combined with fluorescence in situ hybridization(FISH) it is possible to analyze spatial relationships and changes of specific membersof microbial populations over time. The aim of this study was to perform a systematicdescription of the pattern of initial dental biofilm formation by applying 16S rRNA-targeted oligonucleotide probes to the identification of streptococci and other bacteria,and to evaluate the usefulness of the combination of CLSM and FISH for structuralstudies of bacterial populations in dental biofilm. Biofilms were collected on stan-dardized glass slabs mounted in intra-oral appliances and worn by 10 individuals for 6,12, 24 or 48 h. After intra-oral exposure the biofilms were labelled with probes againsteither streptococci (STR405) or all bacteria (EUB338) and analysed by CLSM. Thecurrent approach of using FISH techniques enabled differentiation of streptococcifrom other bacteria and determination of their spatio-temporal organization. Thepresence of chimney-like multilayered microcolonies with different microbial compo-sitions demonstrated by this methodology provided information supplementary to ourprevious knowledge obtained by classical electron microscopic methods and increasedour understanding of the structure of developing biofilms.

KW - biofilm

KW - confocal laser scanning

KW - dental plaque;

KW - fluorescence in situ

KW - ultrastructure

M3 - Journal article

VL - 115

SP - 459

EP - 467

JO - European Journal of Oral Sciences

JF - European Journal of Oral Sciences

SN - 0909-8836

ER -