Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review
In situ identification of streptococci and other bacteria in initial dental biofilm by confocal laser scanning microscopy and fluorescence in situ hybridization. / Dige, Irene; Kilian, Mogens; Nilsson, Holger et al.
I: European Journal of Oral Sciences, Bind 115, 2007, s. 459-467.Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - In situ identification of streptococci and other bacteria in initial dental biofilm by confocal laser scanning microscopy and fluorescence in situ hybridization
AU - Dige, Irene
AU - Kilian, Mogens
AU - Nilsson, Holger
AU - Nyvad, Bente
PY - 2007
Y1 - 2007
N2 - Confocal laser scanning microscopy (CLSM) has been employed as a method forstudying intact natural biofilm. When combined with fluorescence in situ hybridization(FISH) it is possible to analyze spatial relationships and changes of specific membersof microbial populations over time. The aim of this study was to perform a systematicdescription of the pattern of initial dental biofilm formation by applying 16S rRNA-targeted oligonucleotide probes to the identification of streptococci and other bacteria,and to evaluate the usefulness of the combination of CLSM and FISH for structuralstudies of bacterial populations in dental biofilm. Biofilms were collected on stan-dardized glass slabs mounted in intra-oral appliances and worn by 10 individuals for 6,12, 24 or 48 h. After intra-oral exposure the biofilms were labelled with probes againsteither streptococci (STR405) or all bacteria (EUB338) and analysed by CLSM. Thecurrent approach of using FISH techniques enabled differentiation of streptococcifrom other bacteria and determination of their spatio-temporal organization. Thepresence of chimney-like multilayered microcolonies with different microbial compo-sitions demonstrated by this methodology provided information supplementary to ourprevious knowledge obtained by classical electron microscopic methods and increasedour understanding of the structure of developing biofilms.
AB - Confocal laser scanning microscopy (CLSM) has been employed as a method forstudying intact natural biofilm. When combined with fluorescence in situ hybridization(FISH) it is possible to analyze spatial relationships and changes of specific membersof microbial populations over time. The aim of this study was to perform a systematicdescription of the pattern of initial dental biofilm formation by applying 16S rRNA-targeted oligonucleotide probes to the identification of streptococci and other bacteria,and to evaluate the usefulness of the combination of CLSM and FISH for structuralstudies of bacterial populations in dental biofilm. Biofilms were collected on stan-dardized glass slabs mounted in intra-oral appliances and worn by 10 individuals for 6,12, 24 or 48 h. After intra-oral exposure the biofilms were labelled with probes againsteither streptococci (STR405) or all bacteria (EUB338) and analysed by CLSM. Thecurrent approach of using FISH techniques enabled differentiation of streptococcifrom other bacteria and determination of their spatio-temporal organization. Thepresence of chimney-like multilayered microcolonies with different microbial compo-sitions demonstrated by this methodology provided information supplementary to ourprevious knowledge obtained by classical electron microscopic methods and increasedour understanding of the structure of developing biofilms.
KW - biofilm
KW - confocal laser scanning
KW - dental plaque;
KW - fluorescence in situ
KW - ultrastructure
M3 - Journal article
VL - 115
SP - 459
EP - 467
JO - European Journal of Oral Sciences
JF - European Journal of Oral Sciences
SN - 0909-8836
ER -