Trine Bertelsen

The role of mitogen- and stress-activated protein kinase 1 and 2 in chronic skin inflammation in mice

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The role of mitogen- and stress-activated protein kinase 1 and 2 in chronic skin inflammation in mice. / Bertelsen, Trine; Iversen, Lars; Riis, Jette Lindorff; Arthur, J Simon C; Bibby, Bo Martin; Kragballe, Knud; Johansen, Claus.

I: Experimental Dermatology, Bind 20, Nr. 2, 01.02.2011, s. 140-5.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Bertelsen, Trine ; Iversen, Lars ; Riis, Jette Lindorff ; Arthur, J Simon C ; Bibby, Bo Martin ; Kragballe, Knud ; Johansen, Claus. / The role of mitogen- and stress-activated protein kinase 1 and 2 in chronic skin inflammation in mice. I: Experimental Dermatology. 2011 ; Bind 20, Nr. 2. s. 140-5.

Bibtex

@article{3ad061e57a9845ad8566aa7b00c1e5c9,
title = "The role of mitogen- and stress-activated protein kinase 1 and 2 in chronic skin inflammation in mice",
abstract = "Mitogen- and stress-activated protein kinase 1 and 2 (MSK1/2) are two kinases phosphorylated by both ERK1/2 and p38 MAPK. Recently, MSK1 and 2 have been reported to act as negative regulators of acute inflammation. In this study, we investigated the role of MSK1/2 in chronic skin inflammation using an oxazolone-induced allergic contact dermatitis model in MSK1/2 knockout mice and wild-type mice. MSK1/2 knockout mice were demonstrated to have significantly increased inflammation compared with wild-type mice. This was measured by an increased ear thickness, elevated infiltration of neutrophils in the skin and increased inflammatory histological changes. Furthermore, we found significantly elevated levels of the proinflammatory cytokines Tumor necrosis factor-α (TNF-α), IL-1β and IL-6 at both mRNA and protein levels in MSK1/2 knockout mice compared with wild-type mice after oxazolone treatment. In addition, the mRNA expression of the chemokine Thymus and activation regulated chemokine (TARC) was demonstrated to be significantly elevated in oxazolone-treated MSK1/2 knockout mice compared with wild-type mice. The increased expression of TARC was paralleled by increased infiltration of cells positive for the TARC receptor, CCR4, in the dermis of MSK1/2 knockout mice. Our results indicate that MSK1/2 are involved in the activation of feedback mechanisms that dampen oxazolone-induced skin inflammation.",
author = "Trine Bertelsen and Lars Iversen and Riis, {Jette Lindorff} and Arthur, {J Simon C} and Bibby, {Bo Martin} and Knud Kragballe and Claus Johansen",
note = "{\textcopyright} 2011 John Wiley & Sons A/S.",
year = "2011",
month = feb,
day = "1",
doi = "10.1111/j.1600-0625.2010.01153.x",
language = "English",
volume = "20",
pages = "140--5",
journal = "Experimental Dermatology",
issn = "0906-6705",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "2",

}

RIS

TY - JOUR

T1 - The role of mitogen- and stress-activated protein kinase 1 and 2 in chronic skin inflammation in mice

AU - Bertelsen, Trine

AU - Iversen, Lars

AU - Riis, Jette Lindorff

AU - Arthur, J Simon C

AU - Bibby, Bo Martin

AU - Kragballe, Knud

AU - Johansen, Claus

N1 - © 2011 John Wiley & Sons A/S.

PY - 2011/2/1

Y1 - 2011/2/1

N2 - Mitogen- and stress-activated protein kinase 1 and 2 (MSK1/2) are two kinases phosphorylated by both ERK1/2 and p38 MAPK. Recently, MSK1 and 2 have been reported to act as negative regulators of acute inflammation. In this study, we investigated the role of MSK1/2 in chronic skin inflammation using an oxazolone-induced allergic contact dermatitis model in MSK1/2 knockout mice and wild-type mice. MSK1/2 knockout mice were demonstrated to have significantly increased inflammation compared with wild-type mice. This was measured by an increased ear thickness, elevated infiltration of neutrophils in the skin and increased inflammatory histological changes. Furthermore, we found significantly elevated levels of the proinflammatory cytokines Tumor necrosis factor-α (TNF-α), IL-1β and IL-6 at both mRNA and protein levels in MSK1/2 knockout mice compared with wild-type mice after oxazolone treatment. In addition, the mRNA expression of the chemokine Thymus and activation regulated chemokine (TARC) was demonstrated to be significantly elevated in oxazolone-treated MSK1/2 knockout mice compared with wild-type mice. The increased expression of TARC was paralleled by increased infiltration of cells positive for the TARC receptor, CCR4, in the dermis of MSK1/2 knockout mice. Our results indicate that MSK1/2 are involved in the activation of feedback mechanisms that dampen oxazolone-induced skin inflammation.

AB - Mitogen- and stress-activated protein kinase 1 and 2 (MSK1/2) are two kinases phosphorylated by both ERK1/2 and p38 MAPK. Recently, MSK1 and 2 have been reported to act as negative regulators of acute inflammation. In this study, we investigated the role of MSK1/2 in chronic skin inflammation using an oxazolone-induced allergic contact dermatitis model in MSK1/2 knockout mice and wild-type mice. MSK1/2 knockout mice were demonstrated to have significantly increased inflammation compared with wild-type mice. This was measured by an increased ear thickness, elevated infiltration of neutrophils in the skin and increased inflammatory histological changes. Furthermore, we found significantly elevated levels of the proinflammatory cytokines Tumor necrosis factor-α (TNF-α), IL-1β and IL-6 at both mRNA and protein levels in MSK1/2 knockout mice compared with wild-type mice after oxazolone treatment. In addition, the mRNA expression of the chemokine Thymus and activation regulated chemokine (TARC) was demonstrated to be significantly elevated in oxazolone-treated MSK1/2 knockout mice compared with wild-type mice. The increased expression of TARC was paralleled by increased infiltration of cells positive for the TARC receptor, CCR4, in the dermis of MSK1/2 knockout mice. Our results indicate that MSK1/2 are involved in the activation of feedback mechanisms that dampen oxazolone-induced skin inflammation.

U2 - 10.1111/j.1600-0625.2010.01153.x

DO - 10.1111/j.1600-0625.2010.01153.x

M3 - Journal article

C2 - 21166721

VL - 20

SP - 140

EP - 145

JO - Experimental Dermatology

JF - Experimental Dermatology

SN - 0906-6705

IS - 2

ER -