Institut for Biomedicin

Tove Christensen

Urokinase receptor. An activation antigen in human T lymphocytes

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Urokinase receptor. An activation antigen in human T lymphocytes. / Nykjaer, A; Møller, Bjarne Kuno; Todd, R F; Christensen, Tove; Andreasen, Peter; Gliemann, J; Petersen, C M.

I: Journal of Immunology, Bind 152, Nr. 2, 1994, s. 505-16.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Nykjaer, A, Møller, BK, Todd, RF, Christensen, T, Andreasen, P, Gliemann, J & Petersen, CM 1994, 'Urokinase receptor. An activation antigen in human T lymphocytes', Journal of Immunology, bind 152, nr. 2, s. 505-16.

APA

Nykjaer, A., Møller, B. K., Todd, R. F., Christensen, T., Andreasen, P., Gliemann, J., & Petersen, C. M. (1994). Urokinase receptor. An activation antigen in human T lymphocytes. Journal of Immunology, 152(2), 505-16.

CBE

Nykjaer A, Møller BK, Todd RF, Christensen T, Andreasen P, Gliemann J, Petersen CM. 1994. Urokinase receptor. An activation antigen in human T lymphocytes. Journal of Immunology. 152(2):505-16.

MLA

Nykjaer, A o.a.. "Urokinase receptor. An activation antigen in human T lymphocytes". Journal of Immunology. 1994, 152(2). 505-16.

Vancouver

Nykjaer A, Møller BK, Todd RF, Christensen T, Andreasen P, Gliemann J o.a. Urokinase receptor. An activation antigen in human T lymphocytes. Journal of Immunology. 1994;152(2):505-16.

Author

Nykjaer, A ; Møller, Bjarne Kuno ; Todd, R F ; Christensen, Tove ; Andreasen, Peter ; Gliemann, J ; Petersen, C M. / Urokinase receptor. An activation antigen in human T lymphocytes. I: Journal of Immunology. 1994 ; Bind 152, Nr. 2. s. 505-16.

Bibtex

@article{d873afba720b4bedade6d85a48277906,
title = "Urokinase receptor. An activation antigen in human T lymphocytes",
abstract = "The ability of activated T lymphocytes to extravasate and reach inflammatory and malignant foci in the tissues is a basic function of cellular immunity. Recent evidence strongly suggests that the urokinase receptor (uPAR) holds a central position in the development of human two-chain urokinase-mediated pericellular proteolysis and matrix degradation, an important element in cell migration. In this report we establish uPAR as a pan T cell activation Ag. As determined by FACS analysis, CD3+ lymphocytes from healthy donors exhibited no significant uPAR expression. In contrast, patients (e.g., HIV-positive donors) showed distinct uPAR expression, confined to HLA-DR+ cells, in up to 80% of all T cells. In vitro activation by PMA caused a rapid up-regulation of membrane uPAR in all healthy donor T cells and was accompanied by enhanced receptor synthesis and elevated uPAR mRNA levels. A similar induction resulted from activation via the TCR/CD3 complex using mitogens (PHA, and Con A), anti-CD3 antibodies, and alloantigen. Receptor expression at single cell level was also modulated by a number of cytokines. IL-2, IL-4 and IL-7 increased uPAR presentation on 20 to 50% of the T cell population, and combined stimulation of bulk cultures demonstrated an additive effect of IL-2 and IL-7, whereas the response to each of the two was inhibited by IL-4. In addition, TGF-beta 1 substantially reduced the uPAR expression in T cell cultures responding to PHA, IL-2, and IL-7. Irrespective of the activating reagent, the T cells appeared to produce the same molecular uPAR species, but the affinity of uPAR expressed in PMA blasts was decreased, presumably because of a differential location at the cell surface. All activated cultures showed co-expression of uPAR and CD25. The finding that the urokinase receptor is an activation Ag may suggest that cell-associated plasminogen activation is involved in extravasation and migration of activated T cells.",
keywords = "Antigens, CD27, Base Sequence, DNA Primers, Gene Expression, Humans, Interleukin-2, Interleukin-4, Interleukin-7, Lymphocyte Activation, Molecular Sequence Data, Phorbol Esters, RNA, Messenger, Receptors, Antigen, T-Cell, Receptors, Cell Surface, Receptors, Urokinase Plasminogen Activator, T-Lymphocyte Subsets, Transforming Growth Factor beta",
author = "A Nykjaer and M{\o}ller, {Bjarne Kuno} and Todd, {R F} and Tove Christensen and Peter Andreasen and J Gliemann and Petersen, {C M}",
year = "1994",
language = "English",
volume = "152",
pages = "505--16",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "2",

}

RIS

TY - JOUR

T1 - Urokinase receptor. An activation antigen in human T lymphocytes

AU - Nykjaer, A

AU - Møller, Bjarne Kuno

AU - Todd, R F

AU - Christensen, Tove

AU - Andreasen, Peter

AU - Gliemann, J

AU - Petersen, C M

PY - 1994

Y1 - 1994

N2 - The ability of activated T lymphocytes to extravasate and reach inflammatory and malignant foci in the tissues is a basic function of cellular immunity. Recent evidence strongly suggests that the urokinase receptor (uPAR) holds a central position in the development of human two-chain urokinase-mediated pericellular proteolysis and matrix degradation, an important element in cell migration. In this report we establish uPAR as a pan T cell activation Ag. As determined by FACS analysis, CD3+ lymphocytes from healthy donors exhibited no significant uPAR expression. In contrast, patients (e.g., HIV-positive donors) showed distinct uPAR expression, confined to HLA-DR+ cells, in up to 80% of all T cells. In vitro activation by PMA caused a rapid up-regulation of membrane uPAR in all healthy donor T cells and was accompanied by enhanced receptor synthesis and elevated uPAR mRNA levels. A similar induction resulted from activation via the TCR/CD3 complex using mitogens (PHA, and Con A), anti-CD3 antibodies, and alloantigen. Receptor expression at single cell level was also modulated by a number of cytokines. IL-2, IL-4 and IL-7 increased uPAR presentation on 20 to 50% of the T cell population, and combined stimulation of bulk cultures demonstrated an additive effect of IL-2 and IL-7, whereas the response to each of the two was inhibited by IL-4. In addition, TGF-beta 1 substantially reduced the uPAR expression in T cell cultures responding to PHA, IL-2, and IL-7. Irrespective of the activating reagent, the T cells appeared to produce the same molecular uPAR species, but the affinity of uPAR expressed in PMA blasts was decreased, presumably because of a differential location at the cell surface. All activated cultures showed co-expression of uPAR and CD25. The finding that the urokinase receptor is an activation Ag may suggest that cell-associated plasminogen activation is involved in extravasation and migration of activated T cells.

AB - The ability of activated T lymphocytes to extravasate and reach inflammatory and malignant foci in the tissues is a basic function of cellular immunity. Recent evidence strongly suggests that the urokinase receptor (uPAR) holds a central position in the development of human two-chain urokinase-mediated pericellular proteolysis and matrix degradation, an important element in cell migration. In this report we establish uPAR as a pan T cell activation Ag. As determined by FACS analysis, CD3+ lymphocytes from healthy donors exhibited no significant uPAR expression. In contrast, patients (e.g., HIV-positive donors) showed distinct uPAR expression, confined to HLA-DR+ cells, in up to 80% of all T cells. In vitro activation by PMA caused a rapid up-regulation of membrane uPAR in all healthy donor T cells and was accompanied by enhanced receptor synthesis and elevated uPAR mRNA levels. A similar induction resulted from activation via the TCR/CD3 complex using mitogens (PHA, and Con A), anti-CD3 antibodies, and alloantigen. Receptor expression at single cell level was also modulated by a number of cytokines. IL-2, IL-4 and IL-7 increased uPAR presentation on 20 to 50% of the T cell population, and combined stimulation of bulk cultures demonstrated an additive effect of IL-2 and IL-7, whereas the response to each of the two was inhibited by IL-4. In addition, TGF-beta 1 substantially reduced the uPAR expression in T cell cultures responding to PHA, IL-2, and IL-7. Irrespective of the activating reagent, the T cells appeared to produce the same molecular uPAR species, but the affinity of uPAR expressed in PMA blasts was decreased, presumably because of a differential location at the cell surface. All activated cultures showed co-expression of uPAR and CD25. The finding that the urokinase receptor is an activation Ag may suggest that cell-associated plasminogen activation is involved in extravasation and migration of activated T cells.

KW - Antigens, CD27

KW - Base Sequence

KW - DNA Primers

KW - Gene Expression

KW - Humans

KW - Interleukin-2

KW - Interleukin-4

KW - Interleukin-7

KW - Lymphocyte Activation

KW - Molecular Sequence Data

KW - Phorbol Esters

KW - RNA, Messenger

KW - Receptors, Antigen, T-Cell

KW - Receptors, Cell Surface

KW - Receptors, Urokinase Plasminogen Activator

KW - T-Lymphocyte Subsets

KW - Transforming Growth Factor beta

M3 - Journal article

C2 - 8283034

VL - 152

SP - 505

EP - 516

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 2

ER -