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Tove Christensen

Reverse transcriptase activity and particle production in B lymphoblastoid cell lines established from lymphocytes of patients with multiple sclerosis

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Reverse transcriptase activity and particle production in B lymphoblastoid cell lines established from lymphocytes of patients with multiple sclerosis. / Christensen, Tove; Tönjes, R R; zur Megede, J; Boller, K; Møller-Larsen, A.

I: AIDS Research and Human Retroviruses, Bind 15, Nr. 3, 1999, s. 285-91.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{b2bfacfd2e06462584f76a15f1510705,
title = "Reverse transcriptase activity and particle production in B lymphoblastoid cell lines established from lymphocytes of patients with multiple sclerosis",
abstract = "We have established spontaneously formed B lymphoblastoid cell lines from long-term cultured peripheral blood mononuclear cells (PBMNCs) from multiple sclerosis (MS) patients. The MS cell lines actively produce retrovirus-like particles as well as Epstein-Barr virus (EBV). Using three different variations of the highly sensitive polymerase chain reaction (PCR)-based assays for the detection of reverse transcriptase (RT) activity, we have verified the retroviral origin of the retrovirus-like particles that are produced in very low amounts by the MS cell lines.",
keywords = "B-Lymphocytes, Cell Line, Herpesvirus 4, Human, Humans, Microscopy, Electron, Multiple Sclerosis, RNA-Directed DNA Polymerase, Retroviridae, Reverse Transcriptase Polymerase Chain Reaction, Virion",
author = "Tove Christensen and T{\"o}njes, {R R} and {zur Megede}, J and K Boller and A M{\o}ller-Larsen",
year = "1999",
doi = "10.1089/088922299311466",
language = "English",
volume = "15",
pages = "285--91",
journal = "AIDS Research and Human Retroviruses",
issn = "0889-2229",
publisher = "Mary AnnLiebert, Inc. Publishers",
number = "3",

}

RIS

TY - JOUR

T1 - Reverse transcriptase activity and particle production in B lymphoblastoid cell lines established from lymphocytes of patients with multiple sclerosis

AU - Christensen, Tove

AU - Tönjes, R R

AU - zur Megede, J

AU - Boller, K

AU - Møller-Larsen, A

PY - 1999

Y1 - 1999

N2 - We have established spontaneously formed B lymphoblastoid cell lines from long-term cultured peripheral blood mononuclear cells (PBMNCs) from multiple sclerosis (MS) patients. The MS cell lines actively produce retrovirus-like particles as well as Epstein-Barr virus (EBV). Using three different variations of the highly sensitive polymerase chain reaction (PCR)-based assays for the detection of reverse transcriptase (RT) activity, we have verified the retroviral origin of the retrovirus-like particles that are produced in very low amounts by the MS cell lines.

AB - We have established spontaneously formed B lymphoblastoid cell lines from long-term cultured peripheral blood mononuclear cells (PBMNCs) from multiple sclerosis (MS) patients. The MS cell lines actively produce retrovirus-like particles as well as Epstein-Barr virus (EBV). Using three different variations of the highly sensitive polymerase chain reaction (PCR)-based assays for the detection of reverse transcriptase (RT) activity, we have verified the retroviral origin of the retrovirus-like particles that are produced in very low amounts by the MS cell lines.

KW - B-Lymphocytes

KW - Cell Line

KW - Herpesvirus 4, Human

KW - Humans

KW - Microscopy, Electron

KW - Multiple Sclerosis

KW - RNA-Directed DNA Polymerase

KW - Retroviridae

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Virion

U2 - 10.1089/088922299311466

DO - 10.1089/088922299311466

M3 - Journal article

C2 - 10052759

VL - 15

SP - 285

EP - 291

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

SN - 0889-2229

IS - 3

ER -