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Tove Christensen

Isolation of a retrovirus from multiple sclerosis patients in self-generated Iodixanol gradients

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Isolation of a retrovirus from multiple sclerosis patients in self-generated Iodixanol gradients. / Møller-Larsen, A; Christensen, Tove.

I: Journal of Virological Methods, Bind 73, Nr. 2, 1998, s. 151-61.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{84982325a82c4653b66e29c5f76eb6a0,
title = "Isolation of a retrovirus from multiple sclerosis patients in self-generated Iodixanol gradients",
abstract = "The use of Iodixanol, a relatively new iodinated gradient medium, is described for isolation of a retrovirus, which was harvested from the supernatant of lymphoid cell lines originating from patients with multiple sclerosis (MS). The virus is produced in low amounts and has been shown to be fragile, as manifested in a loss of surface glycoproteins when purified in other gradient media. The gradient fractions were analysed after centrifugation in Iodixanol by incorporation of 3H-UTP, reverse transcriptase (RT) assays and electron microscopy (EM) and it was found that Iodixanol does not cause the degree of damage to the particles observed previously. These more favourable conditions are probably due to low viscosity and almost iso-osmotic conditions even in high concentrations. Furthermore, these advantages go together with higher reproducibility in self-forming gradients, easier handling and shorter centrifugation time. Iodixanol can also be used for preparation of HTLV-1.",
keywords = "Cell Line, Centrifugation, Density Gradient, Human T-lymphotropic virus 1, Humans, Lymphocytes, Microscopy, Electron, Multiple Sclerosis, Retroviridae, Reverse Transcriptase Polymerase Chain Reaction, Triiodobenzoic Acids, Virus Cultivation",
author = "A M{\o}ller-Larsen and Tove Christensen",
year = "1998",
language = "English",
volume = "73",
pages = "151--61",
journal = "Journal of Virological Methods",
issn = "0166-0934",
publisher = "Elsevier BV",
number = "2",

}

RIS

TY - JOUR

T1 - Isolation of a retrovirus from multiple sclerosis patients in self-generated Iodixanol gradients

AU - Møller-Larsen, A

AU - Christensen, Tove

PY - 1998

Y1 - 1998

N2 - The use of Iodixanol, a relatively new iodinated gradient medium, is described for isolation of a retrovirus, which was harvested from the supernatant of lymphoid cell lines originating from patients with multiple sclerosis (MS). The virus is produced in low amounts and has been shown to be fragile, as manifested in a loss of surface glycoproteins when purified in other gradient media. The gradient fractions were analysed after centrifugation in Iodixanol by incorporation of 3H-UTP, reverse transcriptase (RT) assays and electron microscopy (EM) and it was found that Iodixanol does not cause the degree of damage to the particles observed previously. These more favourable conditions are probably due to low viscosity and almost iso-osmotic conditions even in high concentrations. Furthermore, these advantages go together with higher reproducibility in self-forming gradients, easier handling and shorter centrifugation time. Iodixanol can also be used for preparation of HTLV-1.

AB - The use of Iodixanol, a relatively new iodinated gradient medium, is described for isolation of a retrovirus, which was harvested from the supernatant of lymphoid cell lines originating from patients with multiple sclerosis (MS). The virus is produced in low amounts and has been shown to be fragile, as manifested in a loss of surface glycoproteins when purified in other gradient media. The gradient fractions were analysed after centrifugation in Iodixanol by incorporation of 3H-UTP, reverse transcriptase (RT) assays and electron microscopy (EM) and it was found that Iodixanol does not cause the degree of damage to the particles observed previously. These more favourable conditions are probably due to low viscosity and almost iso-osmotic conditions even in high concentrations. Furthermore, these advantages go together with higher reproducibility in self-forming gradients, easier handling and shorter centrifugation time. Iodixanol can also be used for preparation of HTLV-1.

KW - Cell Line

KW - Centrifugation, Density Gradient

KW - Human T-lymphotropic virus 1

KW - Humans

KW - Lymphocytes

KW - Microscopy, Electron

KW - Multiple Sclerosis

KW - Retroviridae

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Triiodobenzoic Acids

KW - Virus Cultivation

M3 - Journal article

C2 - 9766886

VL - 73

SP - 151

EP - 161

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

IS - 2

ER -