Institut for Biomedicin

Tove Christensen

Activated monocytes and markers of inflammation in newly diagnosed multiple sclerosis

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Activated monocytes and markers of inflammation in newly diagnosed multiple sclerosis. / Carstensen, Mikkel; Christensen, Tove; Stilund, Morten Leif Munding; Møller, Holger Jon; Petersen, Eva Lykke; Petersen, Thor.

I: Immunology and Cell Biology, Bind 98, Nr. 7, 08.2020, s. 549-562.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Carstensen, M, Christensen, T, Stilund, MLM, Møller, HJ, Petersen, EL & Petersen, T 2020, 'Activated monocytes and markers of inflammation in newly diagnosed multiple sclerosis', Immunology and Cell Biology, bind 98, nr. 7, s. 549-562. https://doi.org/10.1111/imcb.12337

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Author

Carstensen, Mikkel ; Christensen, Tove ; Stilund, Morten Leif Munding ; Møller, Holger Jon ; Petersen, Eva Lykke ; Petersen, Thor. / Activated monocytes and markers of inflammation in newly diagnosed multiple sclerosis. I: Immunology and Cell Biology. 2020 ; Bind 98, Nr. 7. s. 549-562.

Bibtex

@article{fe9eae9b33db4474bd058c5147fe864b,
title = "Activated monocytes and markers of inflammation in newly diagnosed multiple sclerosis",
abstract = "In multiple sclerosis (MS), the inflammation and demyelination of the central nervous system (CNS) develop in distinct ways. This makes diagnosing patients difficult, imperative to initiating early and proper treatment. Several common features exist, among them a profound infiltration of monocytes into the CNS mediating demyelination and tissue destruction. In the periphery, monocytes are divided into three subsets depending on expression of CD14 and CD16, representing different stages of activation and differentiation. To investigate their involvement in MS, peripheral blood mononuclear cells (PBMCs) from 61 patients with incipient, untreated MS and 22 symptomatic control (SC) patients as well as 6 patients with radiologically isolated syndrome (RIS) were characterized ex vivo. In addition, paired serum and cerebrospinal fluid (CSF) samples were analyzed with a panel of biomarkers. In PBMC samples, we demonstrate decreased levels of nonclassical monocytes with a concomitant significant decrease of human endogenous retrovirus (HERV) H3 envelope epitopes on this monocyte subset compared with SC and RIS. The observed HERV expression is present on nonclassical monocytes irrespective of MS and thus presumably a result of the inflammatory activation. For the other surface markers analyzed, we found significantly decreased expression between classical and nonclassical monocytes. In matched samples of CSF a highly significant increase in levels of soluble markers of activation and inflammation is shown, and notably this is not the case for the serum samples. Of the soluble markers investigated, interleukin (IL)-12/IL-23p40 had the highest discriminatory power in differentiating patients with MS from SC and RIS, almost comparable to the immunoglobulin G index.",
keywords = "HERVs, cytokines, inflammation, monocyte subsets, monocytes, multiple sclerosis",
author = "Mikkel Carstensen and Tove Christensen and Stilund, {Morten Leif Munding} and M{\o}ller, {Holger Jon} and Petersen, {Eva Lykke} and Thor Petersen",
year = "2020",
month = aug,
doi = "10.1111/imcb.12337",
language = "English",
volume = "98",
pages = "549--562",
journal = "Immunology and Cell Biology",
issn = "0818-9641",
publisher = "Nature Publishing Group",
number = "7",

}

RIS

TY - JOUR

T1 - Activated monocytes and markers of inflammation in newly diagnosed multiple sclerosis

AU - Carstensen, Mikkel

AU - Christensen, Tove

AU - Stilund, Morten Leif Munding

AU - Møller, Holger Jon

AU - Petersen, Eva Lykke

AU - Petersen, Thor

PY - 2020/8

Y1 - 2020/8

N2 - In multiple sclerosis (MS), the inflammation and demyelination of the central nervous system (CNS) develop in distinct ways. This makes diagnosing patients difficult, imperative to initiating early and proper treatment. Several common features exist, among them a profound infiltration of monocytes into the CNS mediating demyelination and tissue destruction. In the periphery, monocytes are divided into three subsets depending on expression of CD14 and CD16, representing different stages of activation and differentiation. To investigate their involvement in MS, peripheral blood mononuclear cells (PBMCs) from 61 patients with incipient, untreated MS and 22 symptomatic control (SC) patients as well as 6 patients with radiologically isolated syndrome (RIS) were characterized ex vivo. In addition, paired serum and cerebrospinal fluid (CSF) samples were analyzed with a panel of biomarkers. In PBMC samples, we demonstrate decreased levels of nonclassical monocytes with a concomitant significant decrease of human endogenous retrovirus (HERV) H3 envelope epitopes on this monocyte subset compared with SC and RIS. The observed HERV expression is present on nonclassical monocytes irrespective of MS and thus presumably a result of the inflammatory activation. For the other surface markers analyzed, we found significantly decreased expression between classical and nonclassical monocytes. In matched samples of CSF a highly significant increase in levels of soluble markers of activation and inflammation is shown, and notably this is not the case for the serum samples. Of the soluble markers investigated, interleukin (IL)-12/IL-23p40 had the highest discriminatory power in differentiating patients with MS from SC and RIS, almost comparable to the immunoglobulin G index.

AB - In multiple sclerosis (MS), the inflammation and demyelination of the central nervous system (CNS) develop in distinct ways. This makes diagnosing patients difficult, imperative to initiating early and proper treatment. Several common features exist, among them a profound infiltration of monocytes into the CNS mediating demyelination and tissue destruction. In the periphery, monocytes are divided into three subsets depending on expression of CD14 and CD16, representing different stages of activation and differentiation. To investigate their involvement in MS, peripheral blood mononuclear cells (PBMCs) from 61 patients with incipient, untreated MS and 22 symptomatic control (SC) patients as well as 6 patients with radiologically isolated syndrome (RIS) were characterized ex vivo. In addition, paired serum and cerebrospinal fluid (CSF) samples were analyzed with a panel of biomarkers. In PBMC samples, we demonstrate decreased levels of nonclassical monocytes with a concomitant significant decrease of human endogenous retrovirus (HERV) H3 envelope epitopes on this monocyte subset compared with SC and RIS. The observed HERV expression is present on nonclassical monocytes irrespective of MS and thus presumably a result of the inflammatory activation. For the other surface markers analyzed, we found significantly decreased expression between classical and nonclassical monocytes. In matched samples of CSF a highly significant increase in levels of soluble markers of activation and inflammation is shown, and notably this is not the case for the serum samples. Of the soluble markers investigated, interleukin (IL)-12/IL-23p40 had the highest discriminatory power in differentiating patients with MS from SC and RIS, almost comparable to the immunoglobulin G index.

KW - HERVs

KW - cytokines

KW - inflammation

KW - monocyte subsets

KW - monocytes

KW - multiple sclerosis

UR - http://www.scopus.com/inward/record.url?scp=85085130962&partnerID=8YFLogxK

U2 - 10.1111/imcb.12337

DO - 10.1111/imcb.12337

M3 - Journal article

C2 - 32253768

VL - 98

SP - 549

EP - 562

JO - Immunology and Cell Biology

JF - Immunology and Cell Biology

SN - 0818-9641

IS - 7

ER -