Torben Sigsgaard

Cytokine release from the nasal mucosa and whole blood after experimental exposures to organic dusts

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Cytokine release from the nasal mucosa and whole blood after experimental exposures to organic dusts. / Sigsgaard, Torben; Bonefeld-Jørgensen, E C; Kjaergaard, S K; Mamas, S; Pedersen, O F.

I: European Respiratory Journal, Bind 16, Nr. 1, 2000, s. 140-5.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{57a7952fdc9c41f4aaa849e6cbc6f271,
title = "Cytokine release from the nasal mucosa and whole blood after experimental exposures to organic dusts",
abstract = "The aim of this study was to assess the cytokine response after nasal exposure to organic dusts. In a double blinded, crossover study five garbage workers with occupational airway symptoms and five healthy garbage workers were intranasally exposed to endotoxin (lipopolysaccharide LPS), beta-1,3-D-glucan (GLU), Aspergillus sp., compost or the saline dilute for 15 min. Nasal cavity volume and nasal lavage (NAL) were performed at baseline and 3, 6, 11 h postexposure. NAL was analysed with differential cell counts, cysteinyl-leukotrienes, tumour necrosis factor alpha, interleukin (IL)-1beta, IL-6 and IL-8. A whole blood assay on cytokine-release was performed with LPS and GLU. NAL cytokines neutrophils, lymphocytes and albumin increased significantly at 6 h after LPS exposure. GLU induced an increase in albumin and a slight increase in IL-1beta 6-11 h post exposure. In the WBA a significant increase in all cytokines after exposure to LPS as well as GLU was found. Significantly more cells were seen in NAL of the control group 6 h post LPS exposure. In conclusion lipopolysaccharide is the most potent inducer of inflammation in the nasal mucosa whereas compost and beta-1,3-D-glucan only induce minor changes. This reaction to lipopolysaccharide is attenuated in workers with occupational airway symptoms. In whole blood assay, however, beta-1,3-D-glucan also induces cytokine release, indicating a different protective effect of the nasal mucosa towards lipopolysaccharide and beta-1,3-D-glucan.",
keywords = "Allergens, Aspergillus, Asthma, Cross-Over Studies, Cytokines, Double-Blind Method, Dust, Female, Glucans, Humans, Interleukins, Leukotrienes, Lipopolysaccharides, Male, Nasal Lavage Fluid, Nasal Mucosa, Occupational Diseases, Occupational Exposure, Refuse Disposal, Tumor Necrosis Factor-alpha, beta-Glucans",
author = "Torben Sigsgaard and Bonefeld-J{\o}rgensen, {E C} and Kjaergaard, {S K} and S Mamas and Pedersen, {O F}",
year = "2000",
language = "English",
volume = "16",
pages = "140--5",
journal = "European Respiratory Journal",
issn = "0903-1936",
publisher = "European Respiratory Society",
number = "1",

}

RIS

TY - JOUR

T1 - Cytokine release from the nasal mucosa and whole blood after experimental exposures to organic dusts

AU - Sigsgaard, Torben

AU - Bonefeld-Jørgensen, E C

AU - Kjaergaard, S K

AU - Mamas, S

AU - Pedersen, O F

PY - 2000

Y1 - 2000

N2 - The aim of this study was to assess the cytokine response after nasal exposure to organic dusts. In a double blinded, crossover study five garbage workers with occupational airway symptoms and five healthy garbage workers were intranasally exposed to endotoxin (lipopolysaccharide LPS), beta-1,3-D-glucan (GLU), Aspergillus sp., compost or the saline dilute for 15 min. Nasal cavity volume and nasal lavage (NAL) were performed at baseline and 3, 6, 11 h postexposure. NAL was analysed with differential cell counts, cysteinyl-leukotrienes, tumour necrosis factor alpha, interleukin (IL)-1beta, IL-6 and IL-8. A whole blood assay on cytokine-release was performed with LPS and GLU. NAL cytokines neutrophils, lymphocytes and albumin increased significantly at 6 h after LPS exposure. GLU induced an increase in albumin and a slight increase in IL-1beta 6-11 h post exposure. In the WBA a significant increase in all cytokines after exposure to LPS as well as GLU was found. Significantly more cells were seen in NAL of the control group 6 h post LPS exposure. In conclusion lipopolysaccharide is the most potent inducer of inflammation in the nasal mucosa whereas compost and beta-1,3-D-glucan only induce minor changes. This reaction to lipopolysaccharide is attenuated in workers with occupational airway symptoms. In whole blood assay, however, beta-1,3-D-glucan also induces cytokine release, indicating a different protective effect of the nasal mucosa towards lipopolysaccharide and beta-1,3-D-glucan.

AB - The aim of this study was to assess the cytokine response after nasal exposure to organic dusts. In a double blinded, crossover study five garbage workers with occupational airway symptoms and five healthy garbage workers were intranasally exposed to endotoxin (lipopolysaccharide LPS), beta-1,3-D-glucan (GLU), Aspergillus sp., compost or the saline dilute for 15 min. Nasal cavity volume and nasal lavage (NAL) were performed at baseline and 3, 6, 11 h postexposure. NAL was analysed with differential cell counts, cysteinyl-leukotrienes, tumour necrosis factor alpha, interleukin (IL)-1beta, IL-6 and IL-8. A whole blood assay on cytokine-release was performed with LPS and GLU. NAL cytokines neutrophils, lymphocytes and albumin increased significantly at 6 h after LPS exposure. GLU induced an increase in albumin and a slight increase in IL-1beta 6-11 h post exposure. In the WBA a significant increase in all cytokines after exposure to LPS as well as GLU was found. Significantly more cells were seen in NAL of the control group 6 h post LPS exposure. In conclusion lipopolysaccharide is the most potent inducer of inflammation in the nasal mucosa whereas compost and beta-1,3-D-glucan only induce minor changes. This reaction to lipopolysaccharide is attenuated in workers with occupational airway symptoms. In whole blood assay, however, beta-1,3-D-glucan also induces cytokine release, indicating a different protective effect of the nasal mucosa towards lipopolysaccharide and beta-1,3-D-glucan.

KW - Allergens

KW - Aspergillus

KW - Asthma

KW - Cross-Over Studies

KW - Cytokines

KW - Double-Blind Method

KW - Dust

KW - Female

KW - Glucans

KW - Humans

KW - Interleukins

KW - Leukotrienes

KW - Lipopolysaccharides

KW - Male

KW - Nasal Lavage Fluid

KW - Nasal Mucosa

KW - Occupational Diseases

KW - Occupational Exposure

KW - Refuse Disposal

KW - Tumor Necrosis Factor-alpha

KW - beta-Glucans

M3 - Journal article

VL - 16

SP - 140

EP - 145

JO - European Respiratory Journal

JF - European Respiratory Journal

SN - 0903-1936

IS - 1

ER -