Torben Sigsgaard

Acute effect of glucan-spiked office dust on nasal and pulmonary inflammation in guinea pigs

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Acute effect of glucan-spiked office dust on nasal and pulmonary inflammation in guinea pigs. / Straszek, Sune; Adamcakova-Dodd, Andrea; Nervana, Metwali; Pedersen, Ole Finn; Sigsgaard, Torben; Thorne, Peter Sherman.

I: Journal of Toxicology and Environmental Health. Part A, Bind 70, Nr. 22, 2007, s. 1923-1928.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Straszek, S, Adamcakova-Dodd, A, Nervana, M, Pedersen, OF, Sigsgaard, T & Thorne, PS 2007, 'Acute effect of glucan-spiked office dust on nasal and pulmonary inflammation in guinea pigs', Journal of Toxicology and Environmental Health. Part A, bind 70, nr. 22, s. 1923-1928. https://doi.org/10.1080/15287390701551266

APA

Straszek, S., Adamcakova-Dodd, A., Nervana, M., Pedersen, O. F., Sigsgaard, T., & Thorne, P. S. (2007). Acute effect of glucan-spiked office dust on nasal and pulmonary inflammation in guinea pigs. Journal of Toxicology and Environmental Health. Part A, 70(22), 1923-1928. https://doi.org/10.1080/15287390701551266

CBE

Straszek S, Adamcakova-Dodd A, Nervana M, Pedersen OF, Sigsgaard T, Thorne PS. 2007. Acute effect of glucan-spiked office dust on nasal and pulmonary inflammation in guinea pigs. Journal of Toxicology and Environmental Health. Part A. 70(22):1923-1928. https://doi.org/10.1080/15287390701551266

MLA

Straszek, Sune o.a.. "Acute effect of glucan-spiked office dust on nasal and pulmonary inflammation in guinea pigs". Journal of Toxicology and Environmental Health. Part A. 2007, 70(22). 1923-1928. https://doi.org/10.1080/15287390701551266

Vancouver

Straszek S, Adamcakova-Dodd A, Nervana M, Pedersen OF, Sigsgaard T, Thorne PS. Acute effect of glucan-spiked office dust on nasal and pulmonary inflammation in guinea pigs. Journal of Toxicology and Environmental Health. Part A. 2007;70(22):1923-1928. https://doi.org/10.1080/15287390701551266

Author

Straszek, Sune ; Adamcakova-Dodd, Andrea ; Nervana, Metwali ; Pedersen, Ole Finn ; Sigsgaard, Torben ; Thorne, Peter Sherman. / Acute effect of glucan-spiked office dust on nasal and pulmonary inflammation in guinea pigs. I: Journal of Toxicology and Environmental Health. Part A. 2007 ; Bind 70, Nr. 22. s. 1923-1928.

Bibtex

@article{ee78e8008d3011dcbee902004c4f4f50,
title = "Acute effect of glucan-spiked office dust on nasal and pulmonary inflammation in guinea pigs",
abstract = "The acute effects of pure inhaled glucan on respiratory inflammation remain inconclusive and not sufficiently examined with regards to the simultaneous interaction of glucan, endotoxin (LPS) and house dust in airway inflammation. This study aims at determining effects of simultaneous exposure to office dust and glucan on nasal and pulmonary inflammation. This is relevant for humans with occupational exposure in waste handling and farming and buildings with mold problems.  Office dust collected from Danish offices was spiked with 1{\%} (1-3)-β-glucan (curdlan). Guinea pig nasal cavity volume was measured by acoustic rhinometry (AR) and animals were exposed by inhalation for 4 hr to curdlan spiked dust, unspiked dust, purified air (negative controls) or LPS (positive controls). After exposure (+5 hr) or the following day (+18 hr) measurements were repeated by AR and followed by bronchoalveolar lavage (BAL). Total and differential cell counts, IL-8 in BAL fluid and change in nasal volume was compared between groups. A 5-10{\%} increase in nasal volume was seen for all groups including clean air except for a significant 5{\%} decrease for spiked-dust inhalation (+18 hr). No marked differences were observed in BAL cells or IL-8 except in LPS-exposed controls. The delayed decrease of nasal cavity volume after exposure to glucan spiked dust suggests a slow effect on the upper airways for curdlan and office dust together, though no pulmonary response or direct signs of inflammation were observed. Glucan spiked office dust exposures produced a delayed nasal sub-acute congestion in guinea pigs compared to office dust alone but extrapolated to nasal congestion in humans, paralleling the nasal congestion seen in human volunteers exposed to the same dust, it may not have clinical importance.",
keywords = "akustisk rhinometri, inflammation, marsvin, glucan, endotoxin, Acoustic rhinometry, airway inflammation, endotoxin, glucan, guinea pig",
author = "Sune Straszek and Andrea Adamcakova-Dodd and Metwali Nervana and Pedersen, {Ole Finn} and Torben Sigsgaard and Thorne, {Peter Sherman}",
year = "2007",
doi = "10.1080/15287390701551266",
language = "English",
volume = "70",
pages = "1923--1928",
journal = "Journal of Toxicology and Environmental Health. Part A: Current Issues",
issn = "1528-7394",
publisher = "Taylor & francis",
number = "22",

}

RIS

TY - JOUR

T1 - Acute effect of glucan-spiked office dust on nasal and pulmonary inflammation in guinea pigs

AU - Straszek, Sune

AU - Adamcakova-Dodd, Andrea

AU - Nervana, Metwali

AU - Pedersen, Ole Finn

AU - Sigsgaard, Torben

AU - Thorne, Peter Sherman

PY - 2007

Y1 - 2007

N2 - The acute effects of pure inhaled glucan on respiratory inflammation remain inconclusive and not sufficiently examined with regards to the simultaneous interaction of glucan, endotoxin (LPS) and house dust in airway inflammation. This study aims at determining effects of simultaneous exposure to office dust and glucan on nasal and pulmonary inflammation. This is relevant for humans with occupational exposure in waste handling and farming and buildings with mold problems.  Office dust collected from Danish offices was spiked with 1% (1-3)-β-glucan (curdlan). Guinea pig nasal cavity volume was measured by acoustic rhinometry (AR) and animals were exposed by inhalation for 4 hr to curdlan spiked dust, unspiked dust, purified air (negative controls) or LPS (positive controls). After exposure (+5 hr) or the following day (+18 hr) measurements were repeated by AR and followed by bronchoalveolar lavage (BAL). Total and differential cell counts, IL-8 in BAL fluid and change in nasal volume was compared between groups. A 5-10% increase in nasal volume was seen for all groups including clean air except for a significant 5% decrease for spiked-dust inhalation (+18 hr). No marked differences were observed in BAL cells or IL-8 except in LPS-exposed controls. The delayed decrease of nasal cavity volume after exposure to glucan spiked dust suggests a slow effect on the upper airways for curdlan and office dust together, though no pulmonary response or direct signs of inflammation were observed. Glucan spiked office dust exposures produced a delayed nasal sub-acute congestion in guinea pigs compared to office dust alone but extrapolated to nasal congestion in humans, paralleling the nasal congestion seen in human volunteers exposed to the same dust, it may not have clinical importance.

AB - The acute effects of pure inhaled glucan on respiratory inflammation remain inconclusive and not sufficiently examined with regards to the simultaneous interaction of glucan, endotoxin (LPS) and house dust in airway inflammation. This study aims at determining effects of simultaneous exposure to office dust and glucan on nasal and pulmonary inflammation. This is relevant for humans with occupational exposure in waste handling and farming and buildings with mold problems.  Office dust collected from Danish offices was spiked with 1% (1-3)-β-glucan (curdlan). Guinea pig nasal cavity volume was measured by acoustic rhinometry (AR) and animals were exposed by inhalation for 4 hr to curdlan spiked dust, unspiked dust, purified air (negative controls) or LPS (positive controls). After exposure (+5 hr) or the following day (+18 hr) measurements were repeated by AR and followed by bronchoalveolar lavage (BAL). Total and differential cell counts, IL-8 in BAL fluid and change in nasal volume was compared between groups. A 5-10% increase in nasal volume was seen for all groups including clean air except for a significant 5% decrease for spiked-dust inhalation (+18 hr). No marked differences were observed in BAL cells or IL-8 except in LPS-exposed controls. The delayed decrease of nasal cavity volume after exposure to glucan spiked dust suggests a slow effect on the upper airways for curdlan and office dust together, though no pulmonary response or direct signs of inflammation were observed. Glucan spiked office dust exposures produced a delayed nasal sub-acute congestion in guinea pigs compared to office dust alone but extrapolated to nasal congestion in humans, paralleling the nasal congestion seen in human volunteers exposed to the same dust, it may not have clinical importance.

KW - akustisk rhinometri

KW - inflammation

KW - marsvin

KW - glucan

KW - endotoxin

KW - Acoustic rhinometry

KW - airway inflammation

KW - endotoxin

KW - glucan

KW - guinea pig

U2 - 10.1080/15287390701551266

DO - 10.1080/15287390701551266

M3 - Journal article

VL - 70

SP - 1923

EP - 1928

JO - Journal of Toxicology and Environmental Health. Part A: Current Issues

JF - Journal of Toxicology and Environmental Health. Part A: Current Issues

SN - 1528-7394

IS - 22

ER -