Aarhus University Seal / Aarhus Universitets segl

Tofuko Awori Woyengo

Soybean meal allergenic protein degradation and gut health of piglets fed protease-supplemented diets

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Soybean meal allergenic protein degradation and gut health of piglets fed protease-supplemented diets. / Park, Sangwoo; Lee, Jung Wook; Cowieson, Aaron J.; Pappenberger, Guenter; Woyengo, Tofuko Awori.

I: Journal of Animal Science, Bind 98, Nr. 10, skaa308, 10.2020.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Park, S, Lee, JW, Cowieson, AJ, Pappenberger, G & Woyengo, TA 2020, 'Soybean meal allergenic protein degradation and gut health of piglets fed protease-supplemented diets', Journal of Animal Science, bind 98, nr. 10, skaa308. https://doi.org/10.1093/jas/skaa308

APA

Park, S., Lee, J. W., Cowieson, A. J., Pappenberger, G., & Woyengo, T. A. (2020). Soybean meal allergenic protein degradation and gut health of piglets fed protease-supplemented diets. Journal of Animal Science, 98(10), [skaa308]. https://doi.org/10.1093/jas/skaa308

CBE

Park S, Lee JW, Cowieson AJ, Pappenberger G, Woyengo TA. 2020. Soybean meal allergenic protein degradation and gut health of piglets fed protease-supplemented diets. Journal of Animal Science. 98(10):Article skaa308. https://doi.org/10.1093/jas/skaa308

MLA

Vancouver

Park S, Lee JW, Cowieson AJ, Pappenberger G, Woyengo TA. Soybean meal allergenic protein degradation and gut health of piglets fed protease-supplemented diets. Journal of Animal Science. 2020 okt;98(10). skaa308. https://doi.org/10.1093/jas/skaa308

Author

Park, Sangwoo ; Lee, Jung Wook ; Cowieson, Aaron J. ; Pappenberger, Guenter ; Woyengo, Tofuko Awori. / Soybean meal allergenic protein degradation and gut health of piglets fed protease-supplemented diets. I: Journal of Animal Science. 2020 ; Bind 98, Nr. 10.

Bibtex

@article{345c7a590ff54b5f992dec037b48920b,
title = "Soybean meal allergenic protein degradation and gut health of piglets fed protease-supplemented diets",
abstract = "Two experiments were conducted to determine the effects of protease supplementation on degradation of soybean meal (SBM) allergenic proteins (glycinin and β-conglycinin) and gut health of weaned pigs fed soybean meal-based diets. In experiment 1, 2 SBM samples from 2 different sources were subjected to porcine in vitro gastric degradation to determine the effects of protease (at 15,000 U/kg of feedstuff) on degradation of the soybean allergenic proteins. In experiment 2, 48 weaned pigs (body weight = 6.66 kg) were obtained in 2 batches of 24 pigs each. Pigs were individually housed in metabolic crates and fed 4 diets (12 pigs/diet). The diets were corn-based diet with SBM 1 or SBM 2 without or with protease at 15,000 U/kg of diet in 2 × 2 factorial arrangement. Diets were fed for 10 d and pigs were sacrificed on day 10 for measurement of small intestinal histomorphology, permeability of small intestine mounted in Ussing chambers, and serum concentration of pro-inflammatory cytokines. Two SBM sources (SBM 1 and SBM 2) contained 46.9% or 47.7% CP, 14.0% or 14.6% glycinin, and 9.90% or 10.3% β-conglycinin, respectively. Protease and SBM source did not interact on any of the response criteria measured in the current study. Protease supplementation tended to increase (P = 0.069) the in vitro gastric degradation of glycinin. Protease supplementation tended to reduce (P = 0.099) fluorescein isothiocyanate dextran 4,000 Da (which is a marker probe for intestinal permeability) flow in jejunum, and reduced (P = 0.037) serum TNF-α concentration. Protease did not affect small intestinal histomorphology. In conclusion, protease tended to increase gastric degradation of glycinin and reduce gut permeability, and serum concentration of pro-inflammatory cytokines, indicating that the protease used in the current study can be added to SBM-based diets for weanling pigs to improve gut health.",
keywords = "gut health, protease, soybean meal, weaned pig",
author = "Sangwoo Park and Lee, {Jung Wook} and Cowieson, {Aaron J.} and Guenter Pappenberger and Woyengo, {Tofuko Awori}",
year = "2020",
month = oct,
doi = "10.1093/jas/skaa308",
language = "English",
volume = "98",
journal = "Journal of Animal Science",
issn = "0021-8812",
publisher = "AMER SOC ANIMAL SCIENCE",
number = "10",

}

RIS

TY - JOUR

T1 - Soybean meal allergenic protein degradation and gut health of piglets fed protease-supplemented diets

AU - Park, Sangwoo

AU - Lee, Jung Wook

AU - Cowieson, Aaron J.

AU - Pappenberger, Guenter

AU - Woyengo, Tofuko Awori

PY - 2020/10

Y1 - 2020/10

N2 - Two experiments were conducted to determine the effects of protease supplementation on degradation of soybean meal (SBM) allergenic proteins (glycinin and β-conglycinin) and gut health of weaned pigs fed soybean meal-based diets. In experiment 1, 2 SBM samples from 2 different sources were subjected to porcine in vitro gastric degradation to determine the effects of protease (at 15,000 U/kg of feedstuff) on degradation of the soybean allergenic proteins. In experiment 2, 48 weaned pigs (body weight = 6.66 kg) were obtained in 2 batches of 24 pigs each. Pigs were individually housed in metabolic crates and fed 4 diets (12 pigs/diet). The diets were corn-based diet with SBM 1 or SBM 2 without or with protease at 15,000 U/kg of diet in 2 × 2 factorial arrangement. Diets were fed for 10 d and pigs were sacrificed on day 10 for measurement of small intestinal histomorphology, permeability of small intestine mounted in Ussing chambers, and serum concentration of pro-inflammatory cytokines. Two SBM sources (SBM 1 and SBM 2) contained 46.9% or 47.7% CP, 14.0% or 14.6% glycinin, and 9.90% or 10.3% β-conglycinin, respectively. Protease and SBM source did not interact on any of the response criteria measured in the current study. Protease supplementation tended to increase (P = 0.069) the in vitro gastric degradation of glycinin. Protease supplementation tended to reduce (P = 0.099) fluorescein isothiocyanate dextran 4,000 Da (which is a marker probe for intestinal permeability) flow in jejunum, and reduced (P = 0.037) serum TNF-α concentration. Protease did not affect small intestinal histomorphology. In conclusion, protease tended to increase gastric degradation of glycinin and reduce gut permeability, and serum concentration of pro-inflammatory cytokines, indicating that the protease used in the current study can be added to SBM-based diets for weanling pigs to improve gut health.

AB - Two experiments were conducted to determine the effects of protease supplementation on degradation of soybean meal (SBM) allergenic proteins (glycinin and β-conglycinin) and gut health of weaned pigs fed soybean meal-based diets. In experiment 1, 2 SBM samples from 2 different sources were subjected to porcine in vitro gastric degradation to determine the effects of protease (at 15,000 U/kg of feedstuff) on degradation of the soybean allergenic proteins. In experiment 2, 48 weaned pigs (body weight = 6.66 kg) were obtained in 2 batches of 24 pigs each. Pigs were individually housed in metabolic crates and fed 4 diets (12 pigs/diet). The diets were corn-based diet with SBM 1 or SBM 2 without or with protease at 15,000 U/kg of diet in 2 × 2 factorial arrangement. Diets were fed for 10 d and pigs were sacrificed on day 10 for measurement of small intestinal histomorphology, permeability of small intestine mounted in Ussing chambers, and serum concentration of pro-inflammatory cytokines. Two SBM sources (SBM 1 and SBM 2) contained 46.9% or 47.7% CP, 14.0% or 14.6% glycinin, and 9.90% or 10.3% β-conglycinin, respectively. Protease and SBM source did not interact on any of the response criteria measured in the current study. Protease supplementation tended to increase (P = 0.069) the in vitro gastric degradation of glycinin. Protease supplementation tended to reduce (P = 0.099) fluorescein isothiocyanate dextran 4,000 Da (which is a marker probe for intestinal permeability) flow in jejunum, and reduced (P = 0.037) serum TNF-α concentration. Protease did not affect small intestinal histomorphology. In conclusion, protease tended to increase gastric degradation of glycinin and reduce gut permeability, and serum concentration of pro-inflammatory cytokines, indicating that the protease used in the current study can be added to SBM-based diets for weanling pigs to improve gut health.

KW - gut health

KW - protease

KW - soybean meal

KW - weaned pig

UR - http://www.scopus.com/inward/record.url?scp=85093706326&partnerID=8YFLogxK

U2 - 10.1093/jas/skaa308

DO - 10.1093/jas/skaa308

M3 - Journal article

C2 - 32927480

AN - SCOPUS:85093706326

VL - 98

JO - Journal of Animal Science

JF - Journal of Animal Science

SN - 0021-8812

IS - 10

M1 - skaa308

ER -