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Tofuko Awori Woyengo

Gut Microbial Dynamics during Conventionalization of Germfree Chicken

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

DOI

  • Milton Thomas, South Dakota Ctr Biol Res & Commercializat
  • ,
  • Supapit Wongkuna, Mahidol Univ, Mahidol University, Fac Sci, Dept Biochem
  • ,
  • Sudeep Ghimire, South Dakota Ctr Biol Res & Commercializat
  • ,
  • Roshan Kumar, South Dakota Ctr Biol Res & Commercializat
  • ,
  • Linto Antony, South Dakota Ctr Biol Res & Commercializat
  • ,
  • Kinchel C. Doerner, South Dakota State Univ, South Dakota State University, Dept Biol & Microbiol
  • ,
  • Aaron Singery, South Dakota Ctr Biol Res & Commercializat
  • ,
  • Eric Nelson, South Dakota Ctr Biol Res & Commercializat
  • ,
  • Tofuko Woyengo
  • Surang Chankhamhaengdecha, Mahidol Univ, Mahidol University, Fac Sci, Dept Biol
  • ,
  • Tavan Janvilisri, Mahidol Univ, Mahidol University, Fac Sci, Dept Biol
  • ,
  • Joy Scaria, South Dakota Ctr Biol Res & Commercializat

A gnotobiotic Gallus gallus (chicken) model was developed to study the dynamics of intestinal microflora from hatching to 18 days of age employing metagenomics. Intestinal samples were collected from a local population of feral chickens and administered orally to germfree 3-day-old chicks. Animals were euthanized on days 9 and 18 postinoculation, and intestinal samples were collected and subjected to metagenomic analysis. On day 18, the five most prevalent phyla were Bacteroidetes (43.03 +/- 3.19%), Firmicutes (38.51 +/- 2.67%), Actinobacteria (6.77 +/- 0.7%), Proteobacteria (6.38 +/- 0.7%), and Spirochaetes (2.71 +/- 0.55%). Principal-coordinate analysis showed that the day 18 variables clustered more closely than the day 9 variables, suggesting that the microbial communities had changed temporally. The Morista-Horn index values ranged from 0.7 to 1, indicating that the communities in the inoculum and in the day 9 and day 18 samples were more similar than dissimilar. The predicted functional profiles of the microbiomes of the inoculum and the day 9 and day 18 samples were also similar (values of 0.98 to 1). These results indicate that the gnotobiotic chicks stably maintained the phylogenetic diversity and predicted metabolic functionality of the inoculum community.

IMPORTANCE The domestic chicken is the cornerstone of animal agriculture worldwide, with a flock population exceeding 40 billion birds/year. It serves as an economically valuable source of protein globally. The microbiome of poultry has important effects on chicken growth, feed conversion, immune status, and pathogen resistance. The aim of our research was to develop a gnotobiotic chicken model appropriate for the study chicken gut microbiota function. Our experimental model shows that young germfree chicks are able to colonize diverse sets of gut bacteria. Therefore, besides the use of this model to study mechanisms of gut microbiota interactions in the chicken gut, it could be also used for applied aspects such as determining the safety and efficacy of new probiotic strains derived from chicken gut microbiota.

OriginalsprogEngelsk
ArtikelnummerARTN e00035-19
TidsskriftmSphere
Vol/bind4
Nummer2
Antal sider12
ISSN2379-5042
DOI
StatusUdgivet - 2019
Eksternt udgivetJa

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