Institut for Biomedicin

Thomas Vorup-Jensen

Polarization Affects Airway Epithelial Conditioning of Monocyte-Derived Dendritic Cells

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  • Dick Papazian, Ukendt
  • Tashi Chhoden
  • ,
  • Maria Arge, Ukendt
  • Thomas Vorup-Jensen
  • Claus H Nielsen, Danmark
  • Kaare Lund, Ukendt
  • Peter A Würtzen
  • ,
  • Soren Hansen, Danmark

Airway epithelial cells (AECs) form polarized barriers that interact with inhaled allergens and are involved in immune homeostasis. We examined how monocyte-derived dendritic cells (MDDCs) are affected by contact with the airway epithelium. In traditional setups, bronchial epithelial cell lines were allowed to polarize on filter inserts, and MDDCs were allowed to adhere to the epithelial basal side. In an optimized setup, the cell application was reversed, and the culture conditions were modified to preserve cellular polarization and integrity. These two parameters were crucial for the MDDCs' immunoregulatory properties; thus, previous observations obtained using traditional setups should be considered with caution. Using the optimized setup, AEC conditioning of MDDCs led to increased expression of programmed death 1 ligand 1 (PD-L1), Immunoglobulin-Like Transcript 3, CD40, CD80 and CD23. This increased expression was accompanied by decreased secretion of MCP-1 and eotaxin and donor-variable effects on IL-12 and IL-10 secretion. Conditioning varied between maturation states and depended partly on direct contact between AECs and MDDCs. The setup allowed MDDCs on the basal side of the epithelium to sample allergens administered to the apical side. Allergen uptake depended on both polarization and the nature of the allergen. AEC conditioning led to decreased birch allergen-specific proliferation of autologous T cells and a trend toward decreased secretion of the Th2-specific cytokines IL-5 and IL-13. In conclusion, we determined that AEC conditioning favoring cellular integrity leads to a tolerogenic MDDC phenotype, which is likely to be important in regulating immune responses against commonly inhaled allergens.

OriginalsprogEngelsk
TidsskriftAmerican Journal of Respiratory Cell and Molecular Biology (Online)
ISSN1535-4989
DOI
StatusUdgivet - 12 jan. 2015

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