Institut for Biomedicin

Thomas Vorup-Jensen

Challenges and opportunities in fractionation of recombinant human mannan-binding lectin

Publikation: Bidrag til bog/antologi/rapport/proceedingBidrag til bog/antologiForskningpeer review

DOI

Mannan-binding lectin is an oligomeric protein of the innate immune system. Both natural MBL as well as recombinant protein is a heterogeneous composition of oligomers containing from two up to at least eight structural units. Recombinant synthesis in mammalian cell lines often leads to a composition containing small oligomers not abundant in MBL from natural sources such as human plasma. Here, two procedures for fractionation of MBL oligomers are described. In one case, affinity chromatography is employed to enrich the abundance of large MBL oligomers to produce a composition of oligomers resembling natural MBL. In a second case, large-volume ion exchange chromatography is employed to separate these oligomers into fractions with an enhanced size homogeneity. Finally, a well-established procedure for estimating the MBL protein concentration is detailed.

OriginalsprogEngelsk
TitelThe Complement System : Methods and Protocols
Antal sider13
ForlagThe Humana Press Inc.
Udgivelsesår1 jan. 2014
Sider109-121
ISBN (trykt)9781627037235
DOI
StatusUdgivet - 1 jan. 2014
SerietitelMethods in Molecular Biology
Vol/bind1100
ISSN1064-3745

Se relationer på Aarhus Universitet Citationsformater

ID: 153443690