Aarhus University Seal / Aarhus Universitets segl

Thomas Boesen

Crystal structure of the catalytic domain of the initiation factor 2B epsilon subunit from saccharomyces cerevisiae

Publikation: KonferencebidragKonferenceabstrakt til konferenceForskning

  • Molekylærbiologisk Institut
Crystal Structure of Catalytic Domain of the Initiation Factor 2B Epsilon Subunit from Saccharomyces cerevisiae

by

Thomas Boesen1, Graham Pavitt2, and Gregers Rom Andersen1*

1Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK-8000 Århus C, Denmark
2Department of Biomolecular Sciences, UMIST, PO Box 88, Manchester, M60 1QD
UK
*To whom correspondence should be addressed: grand@imsb.au.dk, Tel: (+45) 8942 5024. Fax: (+45) 8612 3178



Abstract

The crystal structure of the C-terminal catalytic domain of the initiation factor 2B epsilon subunit, residues 544-704, from Saccharomyces cerevisiae has been determined to 2.3 Å resolution by the multiple wavelength anomalous dispersion technique using selenomethionine substituted protein. The structure consists of two four helix bundles. The N-terminal four helix bundle contain the catalytic part of the domain, whereas the C-terminal domain harbors the two aa-box motifs involved in binding to the N-terminal part of the initiation factor 2 β subunit. Aliphatic residues in the aa-box motifs are involved in specific contacts in the hydrophobic core of the C-terminal bundle important for maintaining the overall structure, whereas, acidic residues in the motifs form a surface exposed acidic patch which might interact with the lysine boxes of initiation factor 2 β. Interestingly, tryptophan 699 was found to be solvent exposed and involved in crystal packing. This residue could possibly be important for the specific interaction with initiation factor 2 β. Furthermore, the structure shows the location of residues important for catalytic function and disease.

OriginalsprogEngelsk
Udgivelsesår2003
Antal sider1
StatusUdgivet - 2003
BegivenhedTranslation 2003 - , Storbritannien
Varighed: 3 jul. 20034 jul. 2003

Konference

KonferenceTranslation 2003
LandStorbritannien
Periode03/07/200304/07/2003

Bibliografisk note

Sider: T17

Se relationer på Aarhus Universitet Citationsformater

ID: 3233005