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Steen Bønløkke Pedersen

The production and regulation of IGF and IGFBPs in human adipose tissue cultures

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BACKGROUND: Adipocytes express and secrete IGFs and IGFBPs; proteins with important effects on adipocyte homeostasis. However, the factors that control adipocyte generation of IGFs and IGFBPs are not clarified. AIM: To identify regulators of the synthesis of IGFs and IGFBs in adipose tissue. METHODS: Subcutaneous adipose tissue fragments (500mg) from 7 healthy lean women were incubated for 48h following addition of GH (50μg/l), dexamethasone (DXM, 20nM), insulin (100nM), interleukin (IL)-1β (50ng/l), IL-6 (50ng/l) and tumor-necrosis factor (TNF)-α (10ng/l). Outcome parameters included tissue mRNA and culture media IGF and IGFBP levels. RESULTS: Adipose tissue cultures secreted more IGF-II than IGF-I protein (1.14±0.41 vs. 0.26±0.09μg/l [mean±SEM]; P
OriginalsprogEngelsk
TidsskriftGrowth Hormone & I G F Research
Vol/bind22
Nummer6
Sider (fra-til)200-205
Antal sider6
ISSN1096-6374
DOI
StatusUdgivet - dec. 2012

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