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Steen Bønløkke Pedersen

The Expression and Regulation of Bone-acting Cytokines in Human Peripheral Adipose Tissue in Organ Culture

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  • Medicinsk Endokrinologisk Afd., MEA, THG
The humoral cross-talk between bone and fat is an area of increasing interest. We investigated the expression and regulation of the bone-acting cytokines; bone morphogenetic protein 2 (BMP2), connective tissue growth factor (CTGF), osteoprotegerin (OPG), and transforming growth factor beta (TGFB1). Subcutaneous adipose tissue was aspirated from lean, healthy women. Tissue samples were incubated with interleukin 1-β (IL1-β), tumor necrosis factor-α (TNF-α), cortisol, troglitazone, IL1-β + troglitazone, or vehicle. Gene expression in the adipose tissue was analyzed using qPCR and protein levels in the incubation media were analyzed using ELISA. OPG expression and secretion was diminished by 40.8% and 43.1% respectively, by cortisol, and OPG expression was diminished by 67.5% by troglitazone (p
OriginalsprogEngelsk
TidsskriftHormone and Metabolic Research
Vol/bind43
Nummer7
Sider (fra-til)477-82
Antal sider6
ISSN0018-5043
DOI
StatusUdgivet - 1 jun. 2011

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