Aarhus University Seal / Aarhus Universitets segl

Søren Vrønning Hoffmann

Vacuum-ultraviolet circular dichroism reveals DNA duplex formation between short strands of adenine and thymine

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Absorbance spectroscopy is used extensively to tell when two DNA single strands come together and form a double strand. Here we show that circular dichroism in the vacuum ultraviolet region provides an even stronger indication for duplex formation in the case of short strands of adenine and thymine (4 to 16 bases in each strand). Indeed, our results show that a strong positive CD band appears at 179 nm when double strands are formed. Melting experiments were done in aqueous solution with and without added Na counter ions. With additional salt present a huge increase in the 179 nm CD band was observed when lowering the temperature. A 179 nm CD marker band for duplex formation can be used to measure the kinetics for the association of two single strands. Such experiments rely on large changes at one particular wavelength since it is too time-consuming to record a full-wavelength spectrum. This journal is
TidsskriftPhysical Chemistry Chemical Physics
Sider (fra-til)15054-15059
Antal sider6
StatusUdgivet - 2012

Se relationer på Aarhus Universitet Citationsformater

ID: 52255842