Seven Nazipi Bushi

Prevalence of Flp Pili-Encoding Plasmids in Cutibacterium acnes Isolates Obtained from Prostatic Tissue

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Prevalence of Flp Pili-Encoding Plasmids in Cutibacterium acnes Isolates Obtained from Prostatic Tissue. / Davidsson, Sabina; Carlsson, Jessica; Mölling, Paula et al.

I: Frontiers in Microbiology, Bind 8, 16.11.2017, s. 2241.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Davidsson, S, Carlsson, J, Mölling, P, Gashi, N, Andrén, O, Andersson, S-O, Brzuszkiewicz, E, Poehlein, A, Al-Zeer, MA, Brinkmann, V, Scavenius, C, Nazipi, S, Söderquist, B & Brüggemann, H 2017, 'Prevalence of Flp Pili-Encoding Plasmids in Cutibacterium acnes Isolates Obtained from Prostatic Tissue', Frontiers in Microbiology, bind 8, s. 2241. https://doi.org/10.3389/fmicb.2017.02241

APA

Davidsson, S., Carlsson, J., Mölling, P., Gashi, N., Andrén, O., Andersson, S-O., Brzuszkiewicz, E., Poehlein, A., Al-Zeer, M. A., Brinkmann, V., Scavenius, C., Nazipi, S., Söderquist, B., & Brüggemann, H. (2017). Prevalence of Flp Pili-Encoding Plasmids in Cutibacterium acnes Isolates Obtained from Prostatic Tissue. Frontiers in Microbiology, 8, 2241. https://doi.org/10.3389/fmicb.2017.02241

CBE

Davidsson S, Carlsson J, Mölling P, Gashi N, Andrén O, Andersson S-O, Brzuszkiewicz E, Poehlein A, Al-Zeer MA, Brinkmann V, et al. 2017. Prevalence of Flp Pili-Encoding Plasmids in Cutibacterium acnes Isolates Obtained from Prostatic Tissue. Frontiers in Microbiology. 8:2241. https://doi.org/10.3389/fmicb.2017.02241

MLA

Vancouver

Davidsson S, Carlsson J, Mölling P, Gashi N, Andrén O, Andersson S-O et al. Prevalence of Flp Pili-Encoding Plasmids in Cutibacterium acnes Isolates Obtained from Prostatic Tissue. Frontiers in Microbiology. 2017 nov. 16;8:2241. doi: 10.3389/fmicb.2017.02241

Author

Davidsson, Sabina ; Carlsson, Jessica ; Mölling, Paula et al. / Prevalence of Flp Pili-Encoding Plasmids in Cutibacterium acnes Isolates Obtained from Prostatic Tissue. I: Frontiers in Microbiology. 2017 ; Bind 8. s. 2241.

Bibtex

@article{35e3f7a1918e4e49bd7f7dd0e1cc675b,
title = "Prevalence of Flp Pili-Encoding Plasmids in Cutibacterium acnes Isolates Obtained from Prostatic Tissue",
abstract = "Inflammation is one of the hallmarks of prostate cancer. The origin of inflammation is unknown, but microbial infections are suspected to play a role. In previous studies, the Gram-positive, low virulent bacterium Cutibacterium (formerly Propionibacterium) acnes was frequently isolated from prostatic tissue. It is unclear if the presence of the bacterium represents a true infection or a contamination. Here we investigated Cutibacterium acnes type II, also called subspecies defendens, which is the most prevalent type among prostatic C. acnes isolates. Genome sequencing of type II isolates identified large plasmids in several genomes. The plasmids are highly similar to previously identified linear plasmids of type I C. acnes strains associated with acne vulgaris. A PCR-based analysis revealed that 28.4% (21 out of 74) of all type II strains isolated from cancerous prostates carry a plasmid. The plasmid shows signatures for conjugative transfer. In addition, it contains a gene locus for tight adherence (tad) that is predicted to encode adhesive Flp (fimbrial low-molecular weight protein) pili. In subsequent experiments a tad locus-encoded putative pilin subunit was identified in the surface-exposed protein fraction of plasmid-positive C. acnes type II strains by mass spectrometry, indicating that the tad locus is functional. Additional plasmid-encoded proteins were detected in the secreted protein fraction, including two signal peptide-harboring proteins; the corresponding genes are specific for type II C. acnes, thus lacking from plasmid-positive type I C. acnes strains. Further support for the presence of Flp pili in C. acnes type II was provided by electron microscopy, revealing cell appendages in tad locus-positive strains. Our study provides new insight in the most prevalent prostatic subspecies of C. acnes, subsp. defendens, and indicates the existence of Flp pili in plasmid-positive strains. Such pili may support colonization and persistent infection of human prostates by C. acnes.",
keywords = "Journal Article",
author = "Sabina Davidsson and Jessica Carlsson and Paula M{\"o}lling and Natyra Gashi and Ove Andr{\'e}n and Swen-Olof Andersson and Elzbieta Brzuszkiewicz and Anja Poehlein and Al-Zeer, {Munir A} and Volker Brinkmann and Carsten Scavenius and Seven Nazipi and Bo S{\"o}derquist and Holger Br{\"u}ggemann",
year = "2017",
month = nov,
day = "16",
doi = "10.3389/fmicb.2017.02241",
language = "English",
volume = "8",
pages = "2241",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A",

}

RIS

TY - JOUR

T1 - Prevalence of Flp Pili-Encoding Plasmids in Cutibacterium acnes Isolates Obtained from Prostatic Tissue

AU - Davidsson, Sabina

AU - Carlsson, Jessica

AU - Mölling, Paula

AU - Gashi, Natyra

AU - Andrén, Ove

AU - Andersson, Swen-Olof

AU - Brzuszkiewicz, Elzbieta

AU - Poehlein, Anja

AU - Al-Zeer, Munir A

AU - Brinkmann, Volker

AU - Scavenius, Carsten

AU - Nazipi, Seven

AU - Söderquist, Bo

AU - Brüggemann, Holger

PY - 2017/11/16

Y1 - 2017/11/16

N2 - Inflammation is one of the hallmarks of prostate cancer. The origin of inflammation is unknown, but microbial infections are suspected to play a role. In previous studies, the Gram-positive, low virulent bacterium Cutibacterium (formerly Propionibacterium) acnes was frequently isolated from prostatic tissue. It is unclear if the presence of the bacterium represents a true infection or a contamination. Here we investigated Cutibacterium acnes type II, also called subspecies defendens, which is the most prevalent type among prostatic C. acnes isolates. Genome sequencing of type II isolates identified large plasmids in several genomes. The plasmids are highly similar to previously identified linear plasmids of type I C. acnes strains associated with acne vulgaris. A PCR-based analysis revealed that 28.4% (21 out of 74) of all type II strains isolated from cancerous prostates carry a plasmid. The plasmid shows signatures for conjugative transfer. In addition, it contains a gene locus for tight adherence (tad) that is predicted to encode adhesive Flp (fimbrial low-molecular weight protein) pili. In subsequent experiments a tad locus-encoded putative pilin subunit was identified in the surface-exposed protein fraction of plasmid-positive C. acnes type II strains by mass spectrometry, indicating that the tad locus is functional. Additional plasmid-encoded proteins were detected in the secreted protein fraction, including two signal peptide-harboring proteins; the corresponding genes are specific for type II C. acnes, thus lacking from plasmid-positive type I C. acnes strains. Further support for the presence of Flp pili in C. acnes type II was provided by electron microscopy, revealing cell appendages in tad locus-positive strains. Our study provides new insight in the most prevalent prostatic subspecies of C. acnes, subsp. defendens, and indicates the existence of Flp pili in plasmid-positive strains. Such pili may support colonization and persistent infection of human prostates by C. acnes.

AB - Inflammation is one of the hallmarks of prostate cancer. The origin of inflammation is unknown, but microbial infections are suspected to play a role. In previous studies, the Gram-positive, low virulent bacterium Cutibacterium (formerly Propionibacterium) acnes was frequently isolated from prostatic tissue. It is unclear if the presence of the bacterium represents a true infection or a contamination. Here we investigated Cutibacterium acnes type II, also called subspecies defendens, which is the most prevalent type among prostatic C. acnes isolates. Genome sequencing of type II isolates identified large plasmids in several genomes. The plasmids are highly similar to previously identified linear plasmids of type I C. acnes strains associated with acne vulgaris. A PCR-based analysis revealed that 28.4% (21 out of 74) of all type II strains isolated from cancerous prostates carry a plasmid. The plasmid shows signatures for conjugative transfer. In addition, it contains a gene locus for tight adherence (tad) that is predicted to encode adhesive Flp (fimbrial low-molecular weight protein) pili. In subsequent experiments a tad locus-encoded putative pilin subunit was identified in the surface-exposed protein fraction of plasmid-positive C. acnes type II strains by mass spectrometry, indicating that the tad locus is functional. Additional plasmid-encoded proteins were detected in the secreted protein fraction, including two signal peptide-harboring proteins; the corresponding genes are specific for type II C. acnes, thus lacking from plasmid-positive type I C. acnes strains. Further support for the presence of Flp pili in C. acnes type II was provided by electron microscopy, revealing cell appendages in tad locus-positive strains. Our study provides new insight in the most prevalent prostatic subspecies of C. acnes, subsp. defendens, and indicates the existence of Flp pili in plasmid-positive strains. Such pili may support colonization and persistent infection of human prostates by C. acnes.

KW - Journal Article

U2 - 10.3389/fmicb.2017.02241

DO - 10.3389/fmicb.2017.02241

M3 - Journal article

C2 - 29201018

VL - 8

SP - 2241

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

ER -