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Sergey Fedosov

Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats

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Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats. / Kornerup, Linda Skibsted; Fedosov, Sergey; Juul, Christian Bredgaard; Greibe, Eva; Heegaard, Christian Würtz; Nexø, Ebba.

I: European Journal of Nutrition, Bind 57, Nr. 4, 4, 06.2018, s. 1459-1469.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Kornerup, Linda Skibsted ; Fedosov, Sergey ; Juul, Christian Bredgaard ; Greibe, Eva ; Heegaard, Christian Würtz ; Nexø, Ebba. / Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats. I: European Journal of Nutrition. 2018 ; Bind 57, Nr. 4. s. 1459-1469.

Bibtex

@article{2cf1054729444eefbb7f4e3632f5bbad,
title = "Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats",
abstract = "Purpose Hydroxocobalamin (HOCbl) is the dominating Cbl form in food, whereas cyanocobalamin (CNCbl) is common in vitamin pills and oral supplements. This study compares single-dose absorption and distribution of oral HO[Co-57] Cbl and CN[Co-57] Cbl in Cbl-deficient and normal rats.Methods Male Wistar rats (7 weeks) were fed a 14-day diet with (n = 15) or without (n = 15) Cbl. We compared the uptakes of HO[Co-57] Cbl (free or bound to bovine transcobalamin) and free CN[Co-57] Cbl administered by gastric gavage (n = 5 in each diet group). Rats were sacrificed after 24 h. Blood, liver, kidney, brain, heart, spleen, intestines, skeletal muscle, 24-h urine and faeces were collected, and the content of [Co-57] Cbl was measured. Endogenous Cbl in tissues and plasma was analysed by routine methods.Results Mean endogenous plasma-Cbl was sevenfold lower in deficient vs. normal rats (190 vs. 1330 pmol/L, p <0.0001). Cbl depletion increased endogenous Cbl ratios (tissue/plasma = k(in)/k(out)) in all organs except for the kidney, where the ratio decreased considerably. Twenty-four-hour accumulation of labelled Cbl showed that HOCbl > CNCbl (liver) and CNCbl > HOCbl (brain, muscle and plasma).Conclusions The Cbl status of rats and the administered Cbl form influence 24-h Cbl accumulation in tissues and plasma.",
keywords = "Cobalamin deficiency, Cyanocobalamin, Hydroxocobalamin, Intestinal absorption, Kinetic modelling",
author = "Kornerup, {Linda Skibsted} and Sergey Fedosov and Juul, {Christian Bredgaard} and Eva Greibe and Heegaard, {Christian W{\"u}rtz} and Ebba Nex{\o}",
year = "2018",
month = jun,
doi = "10.1007/s00394-017-1424-0",
language = "English",
volume = "57",
pages = "1459--1469",
journal = "European Journal of Nutrition",
issn = "1436-6207",
publisher = "Springer Medizin",
number = "4",

}

RIS

TY - JOUR

T1 - Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats

AU - Kornerup, Linda Skibsted

AU - Fedosov, Sergey

AU - Juul, Christian Bredgaard

AU - Greibe, Eva

AU - Heegaard, Christian Würtz

AU - Nexø, Ebba

PY - 2018/6

Y1 - 2018/6

N2 - Purpose Hydroxocobalamin (HOCbl) is the dominating Cbl form in food, whereas cyanocobalamin (CNCbl) is common in vitamin pills and oral supplements. This study compares single-dose absorption and distribution of oral HO[Co-57] Cbl and CN[Co-57] Cbl in Cbl-deficient and normal rats.Methods Male Wistar rats (7 weeks) were fed a 14-day diet with (n = 15) or without (n = 15) Cbl. We compared the uptakes of HO[Co-57] Cbl (free or bound to bovine transcobalamin) and free CN[Co-57] Cbl administered by gastric gavage (n = 5 in each diet group). Rats were sacrificed after 24 h. Blood, liver, kidney, brain, heart, spleen, intestines, skeletal muscle, 24-h urine and faeces were collected, and the content of [Co-57] Cbl was measured. Endogenous Cbl in tissues and plasma was analysed by routine methods.Results Mean endogenous plasma-Cbl was sevenfold lower in deficient vs. normal rats (190 vs. 1330 pmol/L, p <0.0001). Cbl depletion increased endogenous Cbl ratios (tissue/plasma = k(in)/k(out)) in all organs except for the kidney, where the ratio decreased considerably. Twenty-four-hour accumulation of labelled Cbl showed that HOCbl > CNCbl (liver) and CNCbl > HOCbl (brain, muscle and plasma).Conclusions The Cbl status of rats and the administered Cbl form influence 24-h Cbl accumulation in tissues and plasma.

AB - Purpose Hydroxocobalamin (HOCbl) is the dominating Cbl form in food, whereas cyanocobalamin (CNCbl) is common in vitamin pills and oral supplements. This study compares single-dose absorption and distribution of oral HO[Co-57] Cbl and CN[Co-57] Cbl in Cbl-deficient and normal rats.Methods Male Wistar rats (7 weeks) were fed a 14-day diet with (n = 15) or without (n = 15) Cbl. We compared the uptakes of HO[Co-57] Cbl (free or bound to bovine transcobalamin) and free CN[Co-57] Cbl administered by gastric gavage (n = 5 in each diet group). Rats were sacrificed after 24 h. Blood, liver, kidney, brain, heart, spleen, intestines, skeletal muscle, 24-h urine and faeces were collected, and the content of [Co-57] Cbl was measured. Endogenous Cbl in tissues and plasma was analysed by routine methods.Results Mean endogenous plasma-Cbl was sevenfold lower in deficient vs. normal rats (190 vs. 1330 pmol/L, p <0.0001). Cbl depletion increased endogenous Cbl ratios (tissue/plasma = k(in)/k(out)) in all organs except for the kidney, where the ratio decreased considerably. Twenty-four-hour accumulation of labelled Cbl showed that HOCbl > CNCbl (liver) and CNCbl > HOCbl (brain, muscle and plasma).Conclusions The Cbl status of rats and the administered Cbl form influence 24-h Cbl accumulation in tissues and plasma.

KW - Cobalamin deficiency

KW - Cyanocobalamin

KW - Hydroxocobalamin

KW - Intestinal absorption

KW - Kinetic modelling

UR - http://www.scopus.com/inward/record.url?scp=85047274581&partnerID=8YFLogxK

U2 - 10.1007/s00394-017-1424-0

DO - 10.1007/s00394-017-1424-0

M3 - Journal article

C2 - 28321545

VL - 57

SP - 1459

EP - 1469

JO - European Journal of Nutrition

JF - European Journal of Nutrition

SN - 1436-6207

IS - 4

M1 - 4

ER -