Rubens Spin-Neto

Use of micro-computed tomography for the assessment of periapical lesions in small rodents: a systematic review

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisReviewForskningpeer review

DOI

  • Natália G Kalatzis-Sousa, Department of Restorative Dentistry, Araraquara School of Dentistry, UNESP Univ Estadual Paulista, Araraquara, SP, Brazil, Brasilien
  • R Spin-Neto
  • A Wenzel
  • Mário Tanomaru-Filho, Department of Restorative Dentistry, Araraquara School of Dentistry, UNESP Univ Estadual Paulista, Araraquara, SP, Brazil, Brasilien
  • Gisele Faria, Department of Restorative Dentistry, Araraquara School of Dentistry, UNESP Univ Estadual Paulista, Araraquara, SP, Brazil, Brasilien

This systematic review aimed to review the literature on the acquisition-, reconstruction-, and analysis parameters of micro-computed tomography (micro-CT) for the assessment of periapical lesions in rats and mice, and to illustrate the effect of variation in these parameters. The PubMed database was searched from 2000 to January 2015 (English-language publications) for reports on the use of micro-CT to evaluate periapical lesions in rats and mice. QUADAS criteria were used to rate the quality of the studies. To illustrate the effect of variations in acquisition-, reconstruction-, and analysis parameters on images of periapical lesions, micro-CT examination of two hemi-mandibles of mice, with periapical lesions around the first molar was undertaken. Twenty-one studies were identified, which analyzed periapical lesions in rats or mice using micro-CT. According to the QUADAS, no study was classified as high-, seven were classified as moderate-, and 14 as low quality. The effect of variation in parameters was that voxel size may interfere with image sharpness, reconstruction may interfere with image sharpness and contrast, and inadequate plane orientation may alter the size of the periapical lesion. Non-personalized ROIs resulted in areas that were not part of the periapical lesion. Changing the limits of the threshold for bone-tissue visualization increased lesion size. There is no defined protocol for acquiring and analyzing micro-CT images of periapical lesions in rats and mice. Further, acquisition-, reconstruction-, and analysis parameters are not adequately explained, which may compromise the scientific impact of the studies. This article is protected by copyright. All rights reserved.

OriginalsprogEngelsk
TidsskriftInternational Endodontic Journal
Antal sider15
ISSN0143-2885
DOI
StatusUdgivet - 19 mar. 2016

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