Rubens Spin-Neto

Effects of avocado/soybean unsaponifiables (ASU) on the treatment of ligature-induced periodontitis in rats

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • Guilherme José Pimentel Lopes de Oliveira, Universidade Estadual de São Paulo - UNESP, School of Dentistry of Araraquara, Department of Diagnosis and Surgery, Araraquara, SP, Brazil.
  • ,
  • Luiz Guilherme Freitas de Paula, Centro Universitário Unievangélica, Department of Periodontology, Anápolis, GO, Brazil.
  • ,
  • João Antônio Chaves de Souza, Universidade Federal de Goiás - UFG, Dental School, Department of Stomatological Science, Goiânia, GO, Brazil.
  • ,
  • Rubens Spin-Neto
  • Andreas Stavropoulos, Malmo University, Faculty of Odontology, Department of Periodontology and Community Dentistry, Malmo, Sweden.
  • ,
  • Rosemary Adriana Chiérici Marcantonio, Universidade Estadual de São Paulo - UNESP, School of Dentistry of Araraquara, Department of Diagnosis and Surgery, Araraquara, SP, Brazil.

The purpose of this study is to evaluate the effect of the avocado/soybean unsaponifiables (ASU) on the treatment of induced periodontitis in rats. Periodontitis was induced in 84 rats via ligature placement around the second upper molar, which was removed after 7 days, and scaling and root planning (SRP) was performed at this time. Subsequently, the rats were randomly allocated to four groups with 21 animals each: One SRP group in which saline solution was administered (SS), and three groups in which ASU was administered (0.6 g/kg/day), beginning either 7 days before the induction of periodontitis (SRP/ASU-7), on the day of periodontitis induction (SRP/ASU0), or on the day of treatment (SRP/ASU+7). ASU and SS were administered daily by gavage until the sacrifice of the animals (7, 15, and 30 days after SRP). The % bone in the furcation area was evaluated by histomorphometry and micro-CT. The expression of proteins (TRAP, RANKL, and alkaline phosphatase) and mRNA (IL-1β, TNF-α, IL-6, RANKL, and alkaline phosphatase) were evaluated by immunohistochemistry and qPCR. The SRP/ASU+7 group presented a higher percentage of bone fill in the furcation area and higher expression of alkaline phosphatase than in the SRP group (at 7 and 30 days, respectively). The SRP/ASU0 and SRP/ASU+7 groups presented lower expression levels of RANKL mRNA than the SRP and SRP/ASU-7 groups at 15 days. ASU administration on the day of the SRP treatment of the ligature-induced periodontitis promoted subtle beneficial effects on periodontal repair following the treatment of induced periodontitis within the experimental period of 7 days.

OriginalsprogEngelsk
TidsskriftBrazilian Oral Research
Vol/bind31
Sider (fra-til)e28
ISSN1806-8324
DOI
StatusUdgivet - 10 apr. 2017

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