Institut for Biomedicin

Rasmus H. Olesen

Temporal expression pattern of genes during the period of sex differentiation in human embryonic gonads

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DOI

  • Linn S Mamsen
  • ,
  • Emil H Ernst
  • ,
  • Rehannah Borup, Functional Genomics and Reproductive Health Group, Center for Chromosome Stability, Department of Cellular and Molecular Medicine, University of Copenhagen, Blegdamsvej 3B, Copenhagen, Denmark.
  • ,
  • Agnete Larsen
  • Rasmus H Olesen
  • Erik Ernst
  • Richard A Anderson, MRC Centre for Reproductive Health, University of Edinburgh, 47 Little France Crescent, EH16 4TJ, Edinburgh, United Kingdom.
  • ,
  • Stine G Kristensen
  • ,
  • Claus Y Andersen

The precise timing and sequence of changes in expression of key genes and proteins during human sex-differentiation and onset of steroidogenesis was evaluated by whole-genome expression in 67 first trimester human embryonic and fetal ovaries and testis and confirmed by qPCR and immunohistochemistry (IHC). SRY/SOX9 expression initiated in testis around day 40 pc, followed by initiation of AMH and steroidogenic genes required for androgen production at day 53 pc. In ovaries, gene expression of RSPO1, LIN28, FOXL2, WNT2B, and ETV5, were significantly higher than in testis, whereas GLI1 was significantly higher in testis than ovaries. Gene expression was confirmed by IHC for GAGE, SOX9, AMH, CYP17A1, LIN28, WNT2B, ETV5 and GLI1. Gene expression was not associated with the maternal smoking habits. Collectively, a precise temporal determination of changes in expression of key genes involved in human sex-differentiation is defined, with identification of new genes of potential importance.

OriginalsprogEngelsk
TidsskriftScientific Reports
Vol/bind7
Nummer1
Sider (fra-til)15961
ISSN2045-2322
DOI
StatusUdgivet - 21 nov. 2017

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