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Rasmus Bak

Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAs

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DOI

CRISPR/Cas9 is an effective and easy-to-use tool for editing the genome of many human cancer cell lines. However, in some hard-to-transfect cell lines and primary cells, gene editing is more challenging. This protocol details an electroporation-based protocol for the delivery of Cas9 protein from Streptococcus pyogenes complexed with chemically modified sgRNAs. We have found this protocol to work very efficiently in numerous cell lines and primary cells that are difficult to transfect by conventional chemical-based transfection methods.

OriginalsprogEngelsk
TitelCRISPR gene editing : Methods and protocols
RedaktørerYonglun Luo
Antal sider8
UdgivelsesstedNew York
ForlagSpringer Science+Business Media
Udgivelsesår2019
Sider127-134
ISBN (trykt)978-1-4939-9169-3
ISBN (Elektronisk)978-1-4939-9170-9
DOI
StatusUdgivet - 2019
SerietitelMethods in Molecular Biology
Vol/bind1961
ISSN1064-3745

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