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Ping Song
Theranostic poly(lactic-co-glycolic acid) nanoparticle for magnetic resonance/infrared fluorescence bimodal imaging and efficient siRNA delivery to macrophages and its evaluation in a kidney injury model
Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review
DOI
- https://doi.org/10.1016/j.nano.2017.08.007
Forlagets udgivne version
- Chuanxu Yang ,
- Hieu Vu Quang ,
- Dina Michelle Unnerup Husum ,
- Stine Julie Hyldal Tingskov
- Mads Sloth Vinding
- Thomas Nielsen
- Ping Song
- Niels Christian Nielsen
- Rikke Nørregaard
- Jørgen Kjems
- Interdisciplinary Nanoscience Center - INANO-MBG, iNANO-huset
- Institut for Molekylærbiologi og Genetik
- Interdisciplinary Nanoscience Center - INANO-Bioscience, iNANO-huset
- Institut for Klinisk Medicin - Klinisk fysiologi og nuclearmedicin
- Institut for Klinisk Medicin - De Kirurgiske Forskningslaboratorier, SKS
- Interdisciplinary Nanoscience Center - INANO-Kemi, iNANO-huset
- Institut for Klinisk Medicin - Center for Funktionelt Integrativ Neurovidenskab (CFIN)
- Interdisciplinary Nanoscience Center
- Institut for Klinisk Medicin
- Institut for Molekylærbiologi og Genetik - RNA-biologi og -innovation
In this work, a theranostic nanoparticle was developed for multimodal imaging and siRNA delivery. The core of the nanoparticles (NP) was formed by encapsulation of superparamagnetic iron oxides and indocyanine green in a PLGA matrix to serve as a multimodal probe for near-infrared (NIFR) and magnetic resonance (MR) imaging. The surface of the particle was coated with polyethylenimine (PEI) for siRNA delivery. Macrophages efficiently took up the nanoparticles and emitted strong NIFR and MR contrast. When transfected with siRNA targeting the pro-inflammatory enzyme cyclooxygenase-2 (COX-2), significant down-regulation of COX-2 was achieved in activated macrophages. Furthermore, after injection into a unilateral ureteral obstruction (UUO)-induced kidney injury model, NIFR and MRI imaging revealed accumulation of nanoparticles in the injury kidney. In addition, in vivo silencing of COX-2 was achieved by NP/PEI/siCOX-2, which further attenuated kidney injury. Our theranostic platform represents a promising approach for simultaneous diagnosis and treatment of inflammatory diseases.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Nanomedicine: Nanotechnology, Biology and Medicine |
| Vol/bind | 13 |
| Nummer | 8 |
| Sider (fra-til) | 2451–2462 |
| Antal sider | 12 |
| ISSN | 1549-9634 |
| DOI | |
| Status | Udgivet - nov. 2017 |
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