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Philip Francis Thomsen

Seawater environmental DNA reflects seasonality of a coastal fish community

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

DOI

  • Eva Egelyng Sigsgaard
  • Ida Broman Nielsen, Univ Copenhagen, University of Copenhagen, Ctr GeoGenet, Nat Hist Museum Denmark
  • ,
  • Henrik Carl, Univ Copenhagen, University of Copenhagen, Nat Hist Museum Denmark, Sect Evolutionary Genom
  • ,
  • Marcus Anders Krag, Univ Copenhagen, University of Copenhagen, Nat Hist Museum Denmark, Ctr Macroecol Evolut & Climate
  • ,
  • Steen Wilhelm Knudsen
  • Yingchun Xing, Chinese Acad Fishery Sci, Chinese Academy of Fishery Sciences, Nat Resource & Environm Res Ctr
  • ,
  • Tore Hejl Holm-Hansen, Univ Copenhagen, University of Copenhagen, Nat Hist Museum Denmark, Ctr Macroecol Evolut & Climate
  • ,
  • Peter Rask Moller, Univ Copenhagen, University of Copenhagen, Nat Hist Museum Denmark, Sect Evolutionary Genom
  • ,
  • Philip Francis Thomsen

Coastal marine fish populations are in decline due to overfishing, habitat destruction, climate change and invasive species. Seasonal monitoring is important for detecting temporal changes in the composition of fish communities, but current monitoring is often non-existent or limited to annual or semi-annual surveys. In the present study, we investigate the potential of using environmental DNA (eDNA) metabarcoding of seawater samples to detect the seasonal changes in a coastal marine fish community. Water sampling and snorkelling visual census were performed over 1 year (from 23rd of August 2013 to 11th of August 2014) at a temperate coastal habitat in Denmark (55 degrees 45'39 '' N, 12 degrees 35'59 '' E) and compared to long-term data collected over a 7-year period. We used Illumina sequencing of PCR products to demonstrate that seawater eDNA showed compositional changes in accordance with seasonal changes in the fish community. The vast majority of fish diversity observed in the study area by snorkelling was recovered from sequencing, although the overlap between methods varied widely among sampling events. In total, 24 taxa were detected by both methods, while five taxa were only detected using eDNA and three taxa were only detected by snorkelling. A limitation of the applied primers was the lack of resolution to species level in a few diverse families, and varying sequencing depth between samples represents a potential bias. However, our study demonstrates the utility of eDNA for recovering seasonal variation in marine fish communities, knowledge of which is essential for standardised long-term monitoring of marine biodiversity.

OriginalsprogEngelsk
Artikelnummer128
TidsskriftMarine Biology
Vol/bind164
Nummer6
Antal sider15
ISSN0025-3162
DOI
StatusUdgivet - jun. 2017
Eksternt udgivetJa

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