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Philip Francis Thomsen

Critical considerations for the application of environmental DNA methods to detect aquatic species

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisReviewForskningpeer review

DOI

  • Caren S. Goldberg, Washington State Univ, Washington State University, Sch Environm
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  • Cameron R. Turner, EcoSyst Genet LLC EcoSysGen
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  • Kristy Deiner, Univ Notre Dame, University of Notre Dame, Dept Biol Sci
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  • Katy E. Klymus, Univ Toledo, University of Toledo, Lake Erie Ctr
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  • Philip Francis Thomsen
  • Melanie A. Murphy, Univ Wyoming, University of Wyoming, Dept Ecosyst Sci & Management, Program Ecol, Dept 3354
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  • Stephen F. Spear, Orianne Soc
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  • Anna McKee, US Geol Survey, United States Department of the Interior, United States Geological Survey, South Atlantic Water Sci Ctr
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  • Sara J. Oyler-McCance, US Geol Survey, United States Department of the Interior, United States Geological Survey, Ft Collins Sci Ctr
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  • Robert Scott Cornman, US Geol Survey, United States Department of the Interior, United States Geological Survey, Ft Collins Sci Ctr
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  • Matthew B. Laramie, US Geol Survey, United States Geological Survey, United States Department of the Interior, Forest & Rangeland Ecosyst Sci Ctr
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  • Andrew R. Mahon, Cent Michigan Univ, Central Michigan University, Dept Biol, Inst Great Lakes Res
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  • Richard F. Lance, US Army Engineer Res & Dev Ctr, U.S. Army Corps of Engineers, United States Army, United States Department of Defense, U.S. Army Engineer Research & Development Center (ERDC), Environm Lab
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  • David S. Pilliod, US Geol Survey, United States Geological Survey, United States Department of the Interior, Forest & Rangeland Ecosyst Sci Ctr
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  • Katherine M. Strickler, Washington State Univ, Washington State University, Sch Environm
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  • Lisette P. Waits, Univ Idaho, University of Idaho, Fish & Wildlife Sci
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  • Alexander K. Fremier, Washington State Univ, Washington State University, Sch Environm
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  • Teruhiko Takahara, Shimane Univ, Shimane University, Fac Life & Environm Sci
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  • Jelger E. Herder, Reptile Amphibian & Fish Conservat Netherlands RA
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  • Pierre Taberlet, Univ Grenoble 1, Centre National de la Recherche Scientifique (CNRS), University of Savoie, Universite Grenoble Alpes (UGA), Joseph Fourier University, Univ Grenoble Alpes, CNRS, Lab Ecol Alpine LECA,UMR 5553

Species detection using environmental DNA (eDNA) has tremendous potential for contributing to the understanding of the ecology and conservation of aquatic species. Detecting species using eDNA methods, rather than directly sampling the organisms, can reduce impacts on sensitive species and increase the power of field surveys for rare and elusive species. The sensitivity of eDNA methods, however, requires a heightened awareness and attention to quality assurance and quality control protocols. Additionally, the interpretation of eDNA data demands careful consideration of multiple factors. As eDNA methods have grown in application, diverse approaches have been implemented to address these issues. With interest in eDNA continuing to expand, supportive guidelines for undertaking eDNA studies are greatly needed. Environmental DNA researchers from around the world have collaborated to produce this set of guidelines and considerations for implementing eDNA methods to detect aquatic macroorganisms. Critical considerations for study design include preventing contamination in the field and the laboratory, choosing appropriate sample analysis methods, validating assays, testing for sample inhibition and following minimum reporting guidelines. Critical considerations for inference include temporal and spatial processes, limits of correlation of eDNA with abundance, uncertainty of positive and negative results, and potential sources of allochthonous DNA. We present a synthesis of knowledge at this stage for application of this new and powerful detection method.

OriginalsprogEngelsk
TidsskriftMethods in Ecology and Evolution
Vol/bind7
Nummer11
Sider (fra-til)1299-1307
Antal sider9
ISSN2041-210X
DOI
StatusUdgivet - nov. 2016
Eksternt udgivetJa

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