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Peter Sørensen

Transcriptomic analysis of inbreeding depression in cold-sensitive Drosophila melanogaster shows upregulation of the immune response

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Transcriptomic analysis of inbreeding depression in cold-sensitive Drosophila melanogaster shows upregulation of the immune response. / Vermeulen, Cornelis Joseph; Sørensen, Peter; Kirilova Gagalova, K; Loeschcke, Volker.

I: Journal of Evolutionary Biology, Bind 26, Nr. 9, 09.2013, s. 1890-1902.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Vermeulen, Cornelis Joseph ; Sørensen, Peter ; Kirilova Gagalova, K ; Loeschcke, Volker. / Transcriptomic analysis of inbreeding depression in cold-sensitive Drosophila melanogaster shows upregulation of the immune response. I: Journal of Evolutionary Biology. 2013 ; Bind 26, Nr. 9. s. 1890-1902.

Bibtex

@article{aa7f8fa7f2234eb29e76c065928a2dd8,
title = "Transcriptomic analysis of inbreeding depression in cold-sensitive Drosophila melanogaster shows upregulation of the immune response",
abstract = "In sexually reproducing species, increased homozygosity often causes a decline in fitness, called inbreeding depression. Recently, researchers started describing the functional genomic changes that occur during inbreeding, both in benign conditions and under environmental stress. To further this aim, we have performed a genome-wide gene expression study of inbreeding depression, manifesting as cold sensitivity and conditional lethality. Our focus was to describe general patterns of gene expression during inbreeding depression and to identify specific processes affected in our line. There was a clear difference in gene expression between the stressful restrictive environment and the benign permissive environment in both the affected inbred line and the inbred control line. We noted a strong inbreeding-by-environment interaction, whereby virtually all transcriptional differences between lines were found in the restrictive environment. Functional annotation showed enrichment of transcripts coding for serine proteases and their inhibitors (serpins and BPTI/Kunitz family), which indicates activation of the innate immune response. These genes have previously been shown to respond transcriptionally to cold stress, suggesting the conditional lethal effect is associated with an exaggerated cold stress response. The set of differentially expressed genes significantly overlapped with those found in three other studies of inbreeding depression, demonstrating that it is possible to detect a common signature across different genetic backgrounds.",
keywords = "alpha-mannosidase, cold resistance, conditional lethality, gene expression, genotype-by-environment interaction, inbreeding depression, innate immunity, serine proteases, serpins, thermal resistance",
author = "Vermeulen, {Cornelis Joseph} and Peter S{\o}rensen and {Kirilova Gagalova}, K and Volker Loeschcke",
note = "{\textcopyright} 2013 The Authors. Journal of Evolutionary Biology {\textcopyright} 2013 European Society For Evolutionary Biology.",
year = "2013",
month = sep,
doi = "10.1111/jeb.12183",
language = "English",
volume = "26",
pages = "1890--1902",
journal = "Journal of Evolutionary Biology",
issn = "1010-061X",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "9",

}

RIS

TY - JOUR

T1 - Transcriptomic analysis of inbreeding depression in cold-sensitive Drosophila melanogaster shows upregulation of the immune response

AU - Vermeulen, Cornelis Joseph

AU - Sørensen, Peter

AU - Kirilova Gagalova, K

AU - Loeschcke, Volker

N1 - © 2013 The Authors. Journal of Evolutionary Biology © 2013 European Society For Evolutionary Biology.

PY - 2013/9

Y1 - 2013/9

N2 - In sexually reproducing species, increased homozygosity often causes a decline in fitness, called inbreeding depression. Recently, researchers started describing the functional genomic changes that occur during inbreeding, both in benign conditions and under environmental stress. To further this aim, we have performed a genome-wide gene expression study of inbreeding depression, manifesting as cold sensitivity and conditional lethality. Our focus was to describe general patterns of gene expression during inbreeding depression and to identify specific processes affected in our line. There was a clear difference in gene expression between the stressful restrictive environment and the benign permissive environment in both the affected inbred line and the inbred control line. We noted a strong inbreeding-by-environment interaction, whereby virtually all transcriptional differences between lines were found in the restrictive environment. Functional annotation showed enrichment of transcripts coding for serine proteases and their inhibitors (serpins and BPTI/Kunitz family), which indicates activation of the innate immune response. These genes have previously been shown to respond transcriptionally to cold stress, suggesting the conditional lethal effect is associated with an exaggerated cold stress response. The set of differentially expressed genes significantly overlapped with those found in three other studies of inbreeding depression, demonstrating that it is possible to detect a common signature across different genetic backgrounds.

AB - In sexually reproducing species, increased homozygosity often causes a decline in fitness, called inbreeding depression. Recently, researchers started describing the functional genomic changes that occur during inbreeding, both in benign conditions and under environmental stress. To further this aim, we have performed a genome-wide gene expression study of inbreeding depression, manifesting as cold sensitivity and conditional lethality. Our focus was to describe general patterns of gene expression during inbreeding depression and to identify specific processes affected in our line. There was a clear difference in gene expression between the stressful restrictive environment and the benign permissive environment in both the affected inbred line and the inbred control line. We noted a strong inbreeding-by-environment interaction, whereby virtually all transcriptional differences between lines were found in the restrictive environment. Functional annotation showed enrichment of transcripts coding for serine proteases and their inhibitors (serpins and BPTI/Kunitz family), which indicates activation of the innate immune response. These genes have previously been shown to respond transcriptionally to cold stress, suggesting the conditional lethal effect is associated with an exaggerated cold stress response. The set of differentially expressed genes significantly overlapped with those found in three other studies of inbreeding depression, demonstrating that it is possible to detect a common signature across different genetic backgrounds.

KW - alpha-mannosidase

KW - cold resistance

KW - conditional lethality

KW - gene expression

KW - genotype-by-environment interaction

KW - inbreeding depression

KW - innate immunity

KW - serine proteases

KW - serpins

KW - thermal resistance

U2 - 10.1111/jeb.12183

DO - 10.1111/jeb.12183

M3 - Journal article

C2 - 23944235

VL - 26

SP - 1890

EP - 1902

JO - Journal of Evolutionary Biology

JF - Journal of Evolutionary Biology

SN - 1010-061X

IS - 9

ER -