Aarhus University Seal / Aarhus Universitets segl

Peter Sørensen

Genome-wide transcriptional and post-transcriptional regulation of innate immune and defense responses of bovine mammary gland to Staphylococcus aureus

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • Lingzhao Fang, Key Laboratory of Animal Genetics, China Agricultural University
  • ,
  • Yali Hou, Key Laboratory of Genomic and Precision Medicine, Chinese Academy of Sciences
  • ,
  • Jing An, China Agricultural University
  • ,
  • Bingjie Li
  • ,
  • Minyan Song, China Agricultural University
  • ,
  • Xiao Wang, Key Laboratory of Animal Genetics, China Agricultural University
  • ,
  • Peter Sørensen
  • Yichun Dong, China Agricultural University
  • ,
  • Chao Liu, Key Laboratory of Animal Genetics, China Agricultural University
  • ,
  • Yachun Wang, Key Laboratory of Animal Genetics, China Agricultural University
  • ,
  • Huabin Zhu, Chinese Academy of Agricultural Sciences
  • ,
  • Shengli Zhang, Key Laboratory of Animal Genetics, China Agricultural University
  • ,
  • Ying Yu, Key Laboratory of Animal Genetics, China Agricultural University

Staphylococcus aureus (S. aureus) is problematic for lactating mammals and public health. Understanding of mechanisms by which the hosts respond to severe invasion of S. aureus remains elusive. In this study, the genome-wide expression of mRNAs and miRNAs in bovine mammary gland cells were interrogated at 24 h after intra-mammary infection (IMI) with high or low concentrations of S. aureus. Compared to the negative control quarters, 194 highly-confident responsive genes were identified in the quarters with high concentration (109 cfu/mL) of S. aureus, which were predominantly implicated in pathways and biological processes pertaining to innate immune system, such as cytokine-cytokine receptor interaction and inflammatory response. In contrast, only 21 highly-confident genes were significantly differentially expressed in face of low concentration (106 cfu/mL) of S. aureus, which slightly perturbed the cell signaling and invoked corresponding responses like vasoconstriction, indicating limited perturbations and immunological evading. Additionally, the significant up-regulations of bta-mir-223 and bta-mir-21-3p were observed in the quarters infected by high concentration of S. aureus. Network analysis suggested that the two miRNAs' pivotal roles in defending hosts against bacterial infection probably through inhibiting CXCL14 and KIT. The significant down-regulation of CXCL14 was also observed in bovine mammary epithelial cells at 24 h post-infection of S. aureus (108 cfu/mL) in vitro. Integrated analysis with QTL database further suggested 28 genes (e.g., CXCL14, KIT, and SLC4A11) as candidates of bovine mastitis. This study first systematically revealed transcriptional and post-transcriptional responses of bovine mammary gland cells to invading S. aureus in a dosage-dependent pattern, and highlighted a complicated responsive mechanism in a network of miRNA-gene-pathway interplay.

OriginalsprogEngelsk
Artikelnummer193
TidsskriftFrontiers in cellular and infection microbiology
Vol/bind6
Antal sider17
ISSN2235-2988
DOI
StatusUdgivet - 2016

Se relationer på Aarhus Universitet Citationsformater

ID: 108684889