Aarhus University Seal / Aarhus Universitets segl

Peter Sørensen

General and family-specific gene expression responses to viral hemorrhagic septicaemia virus infection in rainbow trout (Oncorhynchus mykiss)

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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General and family-specific gene expression responses to viral hemorrhagic septicaemia virus infection in rainbow trout (Oncorhynchus mykiss). / Jørgensen, Hanne Birgitte Hede; Sørensen, Peter; Cooper, G A; Lorenzen, E; Lorenzen, N; Hansen, Mette H H; Koop, B F; Henryon, Mark.

I: Molecular Immunology, Bind 48, Nr. 8, 04.2011, s. 1046-1058.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Jørgensen, HBH, Sørensen, P, Cooper, GA, Lorenzen, E, Lorenzen, N, Hansen, MHH, Koop, BF & Henryon, M 2011, 'General and family-specific gene expression responses to viral hemorrhagic septicaemia virus infection in rainbow trout (Oncorhynchus mykiss)', Molecular Immunology, bind 48, nr. 8, s. 1046-1058. https://doi.org/10.1016/j.molimm.2011.01.014

APA

Jørgensen, H. B. H., Sørensen, P., Cooper, G. A., Lorenzen, E., Lorenzen, N., Hansen, M. H. H., Koop, B. F., & Henryon, M. (2011). General and family-specific gene expression responses to viral hemorrhagic septicaemia virus infection in rainbow trout (Oncorhynchus mykiss). Molecular Immunology, 48(8), 1046-1058. https://doi.org/10.1016/j.molimm.2011.01.014

CBE

MLA

Vancouver

Author

Jørgensen, Hanne Birgitte Hede ; Sørensen, Peter ; Cooper, G A ; Lorenzen, E ; Lorenzen, N ; Hansen, Mette H H ; Koop, B F ; Henryon, Mark. / General and family-specific gene expression responses to viral hemorrhagic septicaemia virus infection in rainbow trout (Oncorhynchus mykiss). I: Molecular Immunology. 2011 ; Bind 48, Nr. 8. s. 1046-1058.

Bibtex

@article{19610710f16e11df80f4000ea68e967b,
title = "General and family-specific gene expression responses to viral hemorrhagic septicaemia virus infection in rainbow trout (Oncorhynchus mykiss)",
abstract = "The ability of rainbow trout (Oncorhynchus mykiss) to respond successfully to infection by viral hemorrhagic septicaemia virus (VHSV) is expected to involve a large number of biochemical processes. We hypothesized that this would be reflected at the gene expression level in infected fish, and we tested it by examining gene expression levels in the head kidney of trout at a genome-wide scale with a 16K cDNA microarray for salmonids. Expression levels were recorded during 16 days following bath challenge. The challenge experiment included a relatively low susceptibility (32% survival following challenge) and a relatively high susceptibility (18% survival following challenge) trout family that were both split into a group exposed to virus and a non-exposed control group. In total, 939 genes were differentially expressed between infected and non-infected fish (FDR p = 0.05). Five groups of Gene Ontology categories were involved in immune-related processes and over-represented in infected fish: (i) stress and defense response, (ii) NFkappaB signal transduction, (iii) response to non-self, (iv) antigen processing and presentation, and (v) proteasome complexes. The first four categories were also over-represented among the 642 differentially expressed genes in the low-susceptibility trout family but not among the 556 differentially expressed genes in the high-susceptibility trout family. Expression profiles for most immune genes discussed showed increased transcription from day 3 post-challenge. The results suggest that the innate immune system may play an important role in the successful response to VHSV in rainbow trout. In addition, the results indicate that a superior regulation of the transcription of several key innate immune-related genes contribute to the increased survival in resistant fish",
keywords = "Disease resistance, Gene expression profile, Immune response, Salmonid, Host-pathogen interaction, VHSV",
author = "J{\o}rgensen, {Hanne Birgitte Hede} and Peter S{\o}rensen and Cooper, {G A} and E Lorenzen and N Lorenzen and Hansen, {Mette H H} and Koop, {B F} and Mark Henryon",
year = "2011",
month = apr,
doi = "10.1016/j.molimm.2011.01.014",
language = "English",
volume = "48",
pages = "1046--1058",
journal = "Molecular Immunology",
issn = "0161-5890",
publisher = "Pergamon Press",
number = "8",

}

RIS

TY - JOUR

T1 - General and family-specific gene expression responses to viral hemorrhagic septicaemia virus infection in rainbow trout (Oncorhynchus mykiss)

AU - Jørgensen, Hanne Birgitte Hede

AU - Sørensen, Peter

AU - Cooper, G A

AU - Lorenzen, E

AU - Lorenzen, N

AU - Hansen, Mette H H

AU - Koop, B F

AU - Henryon, Mark

PY - 2011/4

Y1 - 2011/4

N2 - The ability of rainbow trout (Oncorhynchus mykiss) to respond successfully to infection by viral hemorrhagic septicaemia virus (VHSV) is expected to involve a large number of biochemical processes. We hypothesized that this would be reflected at the gene expression level in infected fish, and we tested it by examining gene expression levels in the head kidney of trout at a genome-wide scale with a 16K cDNA microarray for salmonids. Expression levels were recorded during 16 days following bath challenge. The challenge experiment included a relatively low susceptibility (32% survival following challenge) and a relatively high susceptibility (18% survival following challenge) trout family that were both split into a group exposed to virus and a non-exposed control group. In total, 939 genes were differentially expressed between infected and non-infected fish (FDR p = 0.05). Five groups of Gene Ontology categories were involved in immune-related processes and over-represented in infected fish: (i) stress and defense response, (ii) NFkappaB signal transduction, (iii) response to non-self, (iv) antigen processing and presentation, and (v) proteasome complexes. The first four categories were also over-represented among the 642 differentially expressed genes in the low-susceptibility trout family but not among the 556 differentially expressed genes in the high-susceptibility trout family. Expression profiles for most immune genes discussed showed increased transcription from day 3 post-challenge. The results suggest that the innate immune system may play an important role in the successful response to VHSV in rainbow trout. In addition, the results indicate that a superior regulation of the transcription of several key innate immune-related genes contribute to the increased survival in resistant fish

AB - The ability of rainbow trout (Oncorhynchus mykiss) to respond successfully to infection by viral hemorrhagic septicaemia virus (VHSV) is expected to involve a large number of biochemical processes. We hypothesized that this would be reflected at the gene expression level in infected fish, and we tested it by examining gene expression levels in the head kidney of trout at a genome-wide scale with a 16K cDNA microarray for salmonids. Expression levels were recorded during 16 days following bath challenge. The challenge experiment included a relatively low susceptibility (32% survival following challenge) and a relatively high susceptibility (18% survival following challenge) trout family that were both split into a group exposed to virus and a non-exposed control group. In total, 939 genes were differentially expressed between infected and non-infected fish (FDR p = 0.05). Five groups of Gene Ontology categories were involved in immune-related processes and over-represented in infected fish: (i) stress and defense response, (ii) NFkappaB signal transduction, (iii) response to non-self, (iv) antigen processing and presentation, and (v) proteasome complexes. The first four categories were also over-represented among the 642 differentially expressed genes in the low-susceptibility trout family but not among the 556 differentially expressed genes in the high-susceptibility trout family. Expression profiles for most immune genes discussed showed increased transcription from day 3 post-challenge. The results suggest that the innate immune system may play an important role in the successful response to VHSV in rainbow trout. In addition, the results indicate that a superior regulation of the transcription of several key innate immune-related genes contribute to the increased survival in resistant fish

KW - Disease resistance

KW - Gene expression profile

KW - Immune response

KW - Salmonid

KW - Host-pathogen interaction

KW - VHSV

U2 - 10.1016/j.molimm.2011.01.014

DO - 10.1016/j.molimm.2011.01.014

M3 - Journal article

C2 - 21345492

VL - 48

SP - 1046

EP - 1058

JO - Molecular Immunology

JF - Molecular Immunology

SN - 0161-5890

IS - 8

ER -