Aarhus University Seal / Aarhus Universitets segl

Olga Agata Andrzejczak

Expression of starch-binding factor CBM20 in barley plastids controls the number of starch granules and the level of CO2 fixation

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review


  • Yingxin Zhong, Nanjing Agricultural University
  • ,
  • Domenico Sagnelli, Københavns Universitet
  • ,
  • Henrik Bak Topbjerg
  • Harald Hasler-Sheetal, Syddansk Universitet
  • ,
  • Olga Agata Andrzejczak
  • Kourosh Hooshmand
  • René Gislum
  • Dong Jiang, National Technique Innovation Center for Regional Wheat Production/Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture/National Engineering and Technology Center for Information Agriculture, Nanjing Agricultural University
  • ,
  • Ian Max Møller
  • Andreas Blennow, Københavns Universitet
  • ,
  • Kim Henrik Hebelstrup

The biosynthesis of starch granules in plant plastids is coordinated by the orchestrated action of transferases, hydrolases, and dikinases. These enzymes either contain starch-binding domain(s) themselves, or are dependent on direct interactions with co-factors containing starch-binding domains. As a means to competitively interfere with existing starch-protein interactions, we expressed the protein module Carbohydrate-Binding Motif 20 (CBM20), which has a very high affinity for starch, ectopically in barley plastids. This interference resulted in an increase in the number of starch granules in chloroplasts and in formation of compound starch granules in grain amyloplasts, which is unusual for barley. More importantly, we observed a photosystem-independent inhibition of CO2 fixation, with a subsequent reduced growth rate and lower accumulation of carbohydrates with effects throughout the metabolome, including lower accumulation of transient leaf starch. Our results demonstrate the importance of endogenous starch-protein interactions for controlling starch granule morphology and number, and plant growth, as substantiated by a metabolic link between starch-protein interactions and control of CO2 fixation in chloroplasts.

TidsskriftJournal of Experimental Botany
Sider (fra-til)234-246
Antal sider13
StatusUdgivet - 2020

Bibliografisk note

© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Se relationer på Aarhus Universitet Citationsformater

ID: 174400925