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Niels Sandal

Seniorforsker


Personal information
Niels Sandal
Born: 24th of April 1956
Gustav Wieds Vej 10, DK-8000 Aarhus C, phone +45 20760042 or +45 87154934 nns@mbg.au.dk

Positions
2014- Work environment supervisor representative
2011- Senior Scientist, Department of Molecular Biology, Aarhus University
2001-2011 Permanent academic staff (AC-TAP), Department of Molecular Biology, Aarhus University
1999-2000 Associate Professor on Department of Molecular and Structural Biology, Aarhus University
1996-1999 Assistant Professor in molecular genetics of Lotus japonicus in Dr. Jens Stougaards group
1989-1996 Post-doc and Assistant Professor at the University of Aarhus
in Prof. Kjeld A. Marckers group. Project: Nematode resistance in sugar beets
Education & training
1998-1999 Visiting scientist on John Innes Center, Norwich, England in Dr. Noel Ellis group
22. October 1990 Ph.D. from the University of Aarhus, Department of Molecular Biology, Supervisor Prof. Kjeld A. Marcker. Thesis: Nodulins and nodulin gene expression
7. September 1984 M. Sc. in Biology and Chemistry from the University of Aarhus Final project in Plant Molecular Biology

Scientific summary:
Legume plants have symbiosis with nitrogen-fixing bacteria. In our group we are isolating and characterizing the plant genes of the model legume Lotus japonicus that are necessary for symbiosis with the bacterial symbiont Mesorhizobium loti.
For this we use mutant isolation, genetic mapping, map based cloning and whole genome sequencing.
Furthermore we have developed the large (more than hundred thousand plant lines) Lotus retrotransposon 1 (LORE1) genetics resource for isolation of mutants with LORE1 insertions in genes of interest. Seeds from this resource can be ordered through the public LotusBase web site.
I have done forward screening of several thousand lines in this population for mutant phenotypes. In addition to symbiotic mutants I recently found two types of variegated mutants (with white sectors on the leaves) in this screening. At least one of these is a new type of variegation mutant (snowy cotyledon 2). In collaboration with experts in photosynthesis the function of the mutated genes is analysed.

76 published original works with a total number of 5749 citations. Selected examples:

SNOWY COTYLEDON 2 promotes chloroplast development and has a role in leaf variegation inboth Lotus japonicus andArabidopsis thaliana. Zagari N., Sandoval-Ibañez O., Sandal N., Su J.,Rodriguez-Concepcion M., Stougaard J., Pribil M., Leister D., andPulido P. Mol. Plant. 10, 721-734 (2017).

The LORE1 insertion mutant resource. Malolepszy, Anna; Mun, Terry; Sandal, Niels; Gupta, Vikas; Dubin, Manu; Urbanski, Dorian; Shah, Niraj; Bachmann, Asger; Fukai, Eigo; Hirakawa, Hideki; Tabata, Satoshi; Nadzieja, Marcin; Markmann, Katharina;; Su, Junyi; Umehara, Yosuke; Soyano, Takashi; Miyahari, Akira; Sato, Shusei; Hayashi, Makoto; Stougaard, Jens; Andersen, Stig Uggerhøj. Plant Journal (2016).

Receptor-mediatedexopolysaccharide perception controls bacterial infection

Kawaharada, Y., Kelly, S.,Nielsen, M. W., Hjuler, C. T., Gysel, K., Muszyński, A., Carlson, R. W., Thygesen, M. B., Sandal, N., Asmussen, M. H., Vinther, M.,Andersen, S. U., Krusell, L., Thirup, S.,Jensen, K. J., Ronson, C. W., Blaise, M., Radutoiu, S.& Stougaard, J. Receptor-mediatedexopolysaccharide perception controls bacterial infection. Nature 523, 308-312 (2015).


Niels Sandal, Haojie Jin, Dulce Nombre Rodriguez-Navarro, Francisco Temprano, Cristina Cvitanich, Andreas Brachmann, Shusei Sato, Masayoshi Kawaguchi, Satoshi Tabata, Martin Parniske, Jose E. Ruiz-Sainz, Stig U. Andersen, Jens Stougaard. A set of Lotus japonicus Gifu x Lotus burttii recombinant inbred lines facilitate map-based cloning and QTL mapping. DNA Res. 19(4):317-23. (2012).

Leïla Tirichine, Niels Sandal, Lene H. Madsen, Simona Radutoiu, Anita S. Albrektsen, Shusei Sato, Erika Asamizu, Satoshi Tabata, Jens Stougaard A Gain-of-Function Mutation in a Cytokinin Receptor Triggers Spontaneous Root Nodule Organogenesis. Science 315, 104-107 (2007).

Leïla Tirichine, Haruko Imaizumi-Anraku, Satoko Yoshida, Yasuhiro Murakami, Lene H. Madsen, Hiroki Miwa, Tomomi Nakagawa, Niels Sandal, Anita S. Albrektsen, Masayoshi Kawaguchi, Allan Downie, Shusei Sato, Satoshi Tabata, Hiroshi Kouchi, Martin Parniske, Shinji Kawasaki, Jens Stougaard. Deregulation of a Ca2+/calmodulin dependent kinase leads to spontaneous nodule development. Nature 441, 1153-1156 (2006).

Radutoiu, S., Madsen, L.H., Madsen, E.B., Felle, H.H., Umehara, Y., Grønlund, M., Sato, S., Nakamura, Y., Tabata, S., Sandal, N.. and Stougaard, J. Plant recognition of symbiotic bacteria requires two LysM receptor-like kinases. Nature 425, 585-592 (2003).

Madsen, E.B., Madsen, L.H., Radutoiu, S., Olbryt, M., Rakwalska, M., Szczyglowski, Sato, S., Kaneko, T., Tabata, S., Sandal, N. and Stougaard, J. A receptor kinase gene of the LysM type is involved in legume perception of rhizobial signals. Nature 425, 637-640 (2003).

Stracke, S., Kistner, C., Yoshida, S, Mulder, L., Sato, S., Kaneko, T., Tabata, S., Sandal, N., Stougaard, J., Szczyglowski, K. & Parniske, M. A plant receptor-like kinase in the development of arbuscular mycorrhiza and the root nodule symbiosis. Nature 417, 959-962 (2002).

Invited speaker:
World Soybean Research Conference VIII 10.-15. 8. 2009 Beijing, China
Characterization of plant symbiotic genes with the help of the model legume Lotus japonicus
1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation Miyazaki, Japan 20.-24. 9. 2010 Towards identification of the spontaneous nodule formation 4 (snf4) gene of Lotus japonicus through map based cloning and whole genome sequencing

- International collaborations
Japan: Yosuke Umehara, Keisuke Yokota, Makoto Hayashi and Eigo Fukai, Tsukuba,
Shusei Sato og Satoshi Tabata, Kazusa DNA Research Institute, Masayoshi Kawaguchi , Ryo Akashi and Masatsugu Hashiguchi , LegumeBase Miyazaki.
Germany: Martin Parniske, LMU München, Pablo Pulido, LMU München.
Spain: Jose Ruiz Sainz, Dulce Nombre Rodriguez, University of Seville.
Manuel Becana, Consejo Superior de Investigaciones Cientıficas, Zaragoza
Brazil: Andrea Pedrosa
England: Trevor Wang, John Innes Centre, Norwich.

Evaluation of others work
Referee for MPMI, Planta, New Phytologist, Plant Physiology, Annals of Botany, Theoretical and Applied Genetics (TAG), Plant Molecular Biology, National Science Foundation, USA (grants)

Leadership, development and collaboration
Member of a program committee: FVFP-09 Bioteknologi fra Ministeriet for Fødevarer, Landbrug og Fiskeri