Niels Jessen

The acute effects of growth hormone in adipose tissue is associated with suppression of antilipolytic signals

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • Katrine L Høyer
  • Morten L Høgild
  • Edward O List, The Edison Biotechnology Institute, Ohio University
  • ,
  • Kevin Y Lee, Ohio University
  • ,
  • Emily Kissinger, Ohio University
  • ,
  • Rita Sharma, Ohio University
  • ,
  • Nils Erik Magnusson
  • ,
  • Vishwajeet Puri, Ohio University
  • ,
  • John J Kopchick, The Edison Biotechnology Institute, Ohio University
  • ,
  • Jens O L Jørgensen
  • Niels Jessen

AIM: Since GH stimulates lipolysis in vivo after a 2-hr lag phase, we studied whether this involves GH signaling and gene expression in adipose tissue (AT).

METHODS: Human subjects (n = 9) each underwent intravenous exposure to GH versus saline with measurement of serum FFA, and GH signaling, gene array, and protein in AT biopsies after 30-120 min. Human data were corroborated in adipose-specific GH receptor knockout (FaGHRKO) mice versus wild-type mice. Expression of candidate genes identified in the array were investigated in 3T3-L1 adipocytes.

RESULTS: GH increased serum FFA and AT phosphorylation of STAT5b in human subjects. This was replicated in wild-type mice, but not in FaGHRKO mice. The array identified 53 GH-regulated genes, and Ingenuity Pathway analysis showed downregulation of PDE3b, an insulin-dependent antilipolytic signal, upregulation of PTEN that inhibits insulin-dependent antilipolysis, and downregulation of G0S2 and RASD1, both encoding antilipolytic proteins. This was confirmed in 3T3-L1 adipocytes, except for PDE3B, including reciprocal effects of GH and insulin on mRNA expression of PTEN, RASD1, and G0S2.

CONCLUSION: (a) GH directly stimulates AT lipolysis in a GHR-dependent manner, (b) this involves suppression of antilipolytic signals at the level of gene expression, (c) the underlying GH signaling pathways remain to be defined.

OriginalsprogEngelsk
Artikelnummere14373
TidsskriftPhysiological Reports
Vol/bind8
Nummer3
Antal sider9
ISSN2051-817X
DOI
StatusUdgivet - feb. 2020

Bibliografisk note

© 2020 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

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