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Niels Jessen

Growth hormone controls lipolysis by regulation of FSP27 expression

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • Rita Sharma, The Diabetes Institute at Ohio University, Ohio University, Athens, OH 45701, USA.
  • ,
  • Quyen Luong, The Diabetes Institute at Ohio University, Ohio University, Athens, OH 45701, USA.
  • ,
  • Vishva M Sharma, The Diabetes Institute at Ohio University, Ohio University, Athens, OH 45701, USA.
  • ,
  • Mitchell Harberson, The Diabetes Institute at Ohio University, Ohio University, Athens, OH 45701, USA.
  • ,
  • Brian Harper, The Diabetes Institute at Ohio University, Ohio University, Athens, OH 45701, USA.
  • ,
  • Andrew Colborn, The Diabetes Institute at Ohio University, Ohio University, Athens, OH 45701, USA.
  • ,
  • Darlene E Berryman, The Diabetes Institute at Ohio University, Ohio University, Athens, OH 45701, USA.
  • ,
  • Niels Jessen
  • Jens Otto Lunde Jørgensen
  • John J Kopchick, Edison Biotechnology Institute, Ohio University, Athens, OH 45701, USA.
  • ,
  • Vishwajeet Puri, The Diabetes Institute at Ohio University, Ohio University, Athens, OH 45701, USA.
  • ,
  • Kevin Y Lee, The Diabetes Institute at Ohio University, Ohio University, Athens, OH 45701, USA.

Growth hormone (GH) has long been known to stimulate lipolysis and insulin resistance; however, the molecular mechanisms underlying these effects are unknown. In the present study, we demonstrate that GH acutely induces lipolysis in cultured adipocytes. This effect is secondary to the reduced expression of a negative regulator of lipolysis, fat-specific protein 27 (FSP27; aka Cidec) at both the mRNA and protein levels. These effects are mimicked in vivo as transgenic overexpression of GH leads to a reduction of FSP27 expression. Mechanistically, we show GH modulation of FSP27 expression is mediated through activation of both MEK/ERK- and STAT5-dependent intracellular signaling. These two molecular pathways interact to differentially manipulate peroxisome proliferator-activated receptor gamma activity (PPARγ) on the FSP27 promoter. Furthermore, overexpression of FSP27 is sufficient to fully suppress GH-induced lipolysis and insulin resistance in cultured adipocytes. Taken together, these data decipher a molecular mechanism by which GH acutely regulates lipolysis and insulin resistance in adipocytes.

OriginalsprogEngelsk
TidsskriftJournal of Endocrinology
Vol/bind239
Nummer3
Sider (fra-til)289-301
Antal sider13
ISSN0022-0795
DOI
StatusUdgivet - 1 dec. 2018

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