Institut for Biomedicin

Mikael Esmann

Bulk properties of the lipid bilayer are not essential for the thermal stability of Na,K-ATPase from shark rectal gland or pig kidney

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  • Institut for Fysiologi og Biofysik
The thermal stability of Na,K-ATPase from pig kidney is markedly greater than that of Na,K-ATPase from shark salt glands. The role of the lipid bilayer is studied by solubilisation of the membrane-bound enzyme in the nonionic detergent octaethyleneglycoldodecylmonoether (C(12)E(8)), addition of excess dioleylphosphatidylcholine (DOPC) or palmitoyloleylphosphatidylcholine (POPC) and reconstitution of membranes by removal of detergent. At 54°C the reconstituted enzymatically active pig enzyme retains a high thermal stability, and reconstituted shark enzyme retains a low thermal stability, even with a 9-fold excess of DOPC. This result suggests that the origin of the difference in thermal stability is not related to bulk lipid properties of the native membranes.
OriginalsprogEngelsk
TidsskriftBiochemical and Biophysical Research Communications
Vol/bind406
Nummer4
Sider (fra-til)580-3
Antal sider4
ISSN0006-291X
DOI
StatusUdgivet - 25 mar. 2011

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